| Project/Area Number |
17591659
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | National Cardiovascular Center |
|---|
Principal Investigator |
YAMAZAKI Toji (2006) National Cardiovascular Center, Dept of Cardiac Physiology, Research staff, 心臓生理部, 室長 (20116122)
高内 裕司 (2005) 国立循環器病センター(研究所), 麻酔科, 医員 (60283751)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AKIYAMA Tsuyoshi National Cardiovascular Center, Dept of Cardiac Physiology, Research staff, 心臓生理部, 室長 (70202554)
山崎 登自 国立循環器病センター(研究所), 心臓生理部, 研究員 (20116122)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | microdialysis / myoglobin / anesthesia / myocardial ischemia / myocardial reperfusion |
|---|
| Research Abstract |
Recently we have developed cardiac microdialysis for detecting of protein leakage from the injured myocardium and monitored myocardial interstitial myoglobin levels as an index of myocardial injury. Using this method, we examined whether the exposures to isoflurane would exert a beneficial effect on myocardial injury evoked by ischemia or reperfusion. Methods : A dialysis probe was implanted into the left ventricle free wall in the pentobarbital-anesthetized rabbits. The dialysate myoglobin level served as an index of myocardial interstitial myoglobin levels. Rabbits were randomly assigned to one of three groups : (1)Vehicle group (n=6) was without exposure to isoflurane (vehicle, n=6). (2)Rabbits were made to inhale 1 MAC isoflurane once for 30-min (ISO30-1, n=6) and (3)twice for 30-min (ISO30-2, n=6), with a 15-min washout period interspersed. Isoflurane exposure was followed by a 15-min washout period before ischemia, and all rabbits then underwent 30 min of coronary occlusion and 60 min of reperfusion. Results : In all three groups dialysate myoglobin levels were increased by coronary occlusion and furthermore augmented by reperfusion. In comparison with the vehicle group, the ISO30-1 group suppressed only the increase in the dialysate myoglobin level during reperfusion. The ISO30-2 group suppressed the increases in dialysate myoglobin levels during both the ischemic and reperfusion periods. Conclusion : Single exposure to isoflurane suppressed myocardial myoglobin release during the reperfusion period but not during the ischemic period. On the other hand, dual exposure to isoflurane suppressed both myocardial myoglobin release evoked by both ischemia and reperfusion injury. Repeated exposure to isoflurane exposure may provide more protection against myocardial ischemia or reperfusion injuries than single exposure.
|
