| Project/Area Number |
17591664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOMITA Kyoichi The University of Tokyo, Hospital, associate professor (20272578)
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| Co-Investigator(Kenkyū-buntansha) |
KUME Haruki The University of Tokyo, Hospital, assistant professor (10272577)
KITAMURA Tadaichi The University of Tokyo, Hospital, Professor (70010551)
有賀 誠司 東京大学, 医学部附属病院, 助手 (90322057)
山田 雄太 東京大学, 医学部附属病院, 助手 (10376452)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,940,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | bladder cancer / invasion / E-cadherin / N-cadherin / prognosis / confocal scanning laser microscopy / cadherin switching / c-Met / 培養細胞 / HGF |
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| Research Abstract |
We investigated the expression of N-cadherin in cultured human bladder cancer cells and human bladder cancer specimens. We aim at establishing the pathological role of induced expression of N-cadherin in bladder cancer progression and show a correlation between expression of cadherins and clinical outcome. In 45 bladder cancer specimens of radical cystectomy or transurethral resection, double-label immunofluorescece staining of both E-and N-cadherin was performed, and patterns of these expressions were evaluated. We investigated to clarify the localization of N-cadherin expression using confocal scanning laser microscopy (CSLM). In cultured cancer cells, we did not confirm to change from E-cadherin to n-cadherin according to hepatocyte growth factor. Normal expression of E-cadherin was found in 20 cases (44.4%), and positive expression of N-cadherin (membranous pattern and/or dotted pattern) was found in 24 cases (53.3%). Aberrant expression of E-cadherin and positive expression of N-cadherin have a significant correlation with tumor stage. The ratio of 5 years survival rate was as follows:E-cadherin (normal 100%: aberrant 59.2%), and N-cadherin (positive 62.5%: negative 94.7%), respectively. There was a significant difference in survival between normal and aberrant expression of E-cadherin, or between positive and negative expression of N-cadherin. Bladder cancer cells invading stroma strongly showed positive N-cadherin expression by CSLM. In bladder cancer patients, there is a significant correlation between E-cadherin and N-cadherin, and pT stage. There is a significant correlation between aberrant expression of E-cadherin and positive expression of N-cadherin. Aberrant expression of E-cadherin and positive expression of N-cadherin show poor prognostic factors in survival of bladder cancer patients. Our data revealed N-cadherin expression may play an important role in bladder cancer invasion and metastasis.
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