Significance and development of Urine uroplakin III mRNA measurement in vesicoureteral reflux
| Project/Area Number |
17591673
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Shiga University of Medical Science |
|---|
Principal Investigator |
JOHNIN Kazuyoshi Shiga University of Medical Science, Faculty of Medicine, The Department of Urology, Assistant (90324590)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIKI Tastuhiro Shiga University of Medical Science, Faculty of Medicine the Department of Urology, Associate Professor (80230704)
NARITA Mitsuhiro Shiga University of Medical Science, Faculty of Medicine the Department of Urology, Associate Professor (00263046)
KAGEYAMA Susumu Shiga University of Medical Science, Faculty of Medicine the Department of Urology, Associate Professor (50378452)
坂野 祐司 滋賀医科大学, 医学部, 助手 (00346016)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | vesicoureteral reflux / uroplakin III / screening / quantitative PCR |
|---|
| Research Abstract |
Vesicoureteral reflux (VUR) is the most common congenital urinary tract anomaly. This disease can pose a major threat to the kidneys as twenty percent of patients with endstage renal disease. are reported to have VUR. Although genetic studies for uroplakin III (UPIII) have been reported recently, no study has focused on UPIII gene expression in VUR patients. We describe here the up-regulation of UPIII mRNA in exfoliated urinary cells from primary VUR patients. A real-time RT-PCR for KRIII mRNA was performed on exfoliated urothelial cells from 18 primary VUR and 38 control samples. UPIII mRNA copies were calculated for each sample. The statistical differences were assessed by the Mann-Whitney U test. Receiver operator characteristic curves were constructed for analysis of the diagnostic values. UPIII mRNA was found to be up-regulated to a greater extent in VUR than in control exfoliated urinary cells (mean ± SE: 497.0±178.5 copies vs. 69.0±10.0 copies, respectively, p<0.00l). In evaluating the measurement of urinary UPIII mRNA as a screening test for VUR, the sensitivity was 77.8% and the specificity was 76.3% by the best diagnostic cutoff point. This is the first report demonstrating up-regulation of UPIII in mRNA levels in VUR patients. We submit that the quantitative measurement of urinary UPIII mRNA has a potential of developing into the first non-invasive screening test for VUR.
|
Report
(3 results)
Research Products
(6 results)
