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New therapy for renal cell carcinoma with down regulation of glycosyltransferase gene

Research Project

Project/Area Number 17591676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionTohoku University

Principal Investigator

ITO Akihiro  Tohoku University Hospital, Research associate, 病院, 助手 (70344661)

Co-Investigator(Kenkyū-buntansha) OGAWA Osamu  Kyoto University, Faculty of medicine, Professor, 医学研究科, 教授 (90260611)
KAMOTO Toshiyuki  Kyoto University, Faculty of medicine, Associate professor, 医学研究科, 助教授 (00281098)
NISHIYAMA Hiroyuki  Kyoto University, Faculty of medicine, Research associate, 医学研究科, 助手 (20324642)
SAITO Seiichi  Tohoku University, Graduate School of medicine, associate professor, 大学院医学系研究科, 助教授 (80235043)
ARAI Yoichi  Tohoku University, Graduate School of medicine, Professor, 大学院医学系研究科, 教授 (50193058)
佐藤 信  東北大学, 大学院医学系研究科, 助教授 (70282134)
中村 英二郎  京都大学, 医学研究科, 助手 (90293878)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsrenal cell carcinoma / glycolipid / ganglioside / Siglec-7 / DSGb5 / St6GalNAcVI / siRNA / cell motility / Silec-7 / siRNA糖鎖合成酵素遺伝子
Research Abstract

We have investigated changes of ganliosides in renal cell carcinoma (ROC) in regard to the malignant potential and found that the long chain gangliosides increased in metastasis, one of which is disialosyl globopentaosylceramide (DSGb5). The purpose of this project is to clarify the functional role of DSGb5. We have identified that 2,6-sialyltransferase (ST6GalNAcVI) catalyzes transfer of sialic acid to monosialosyl globopentaosylceramide (MSGb5) to synthesizes DSGb5. Down-regulation of ST6GalNAcVI mRNA by siRNA has led to decreases of motility of human renal cell carcinoma cells (ACHN cells) in vitro and decreases of cell growth of ACHN in nude mice. From these findings, DSGb5 has relevance to motility and growth of ACHN cells and down-regulation of ST6GalNac VI gene may become a new therapy for RCC.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (6 results)

All 2007 2006

All Journal Article (6 results)

  • [Journal Article] Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells : downregulation of ST6GalNAc VI in renal cancers.2007

    • Author(s)
      Senda M, Ito A, Tsuchida A, Hagiwara T, Kaneda T, Nakamura Y, Frukawa K
    • Journal Title

      Biochem J. 402・3

      Pages: 459-470

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells : downregulation of ST6GalNAc VI in renal cancers.2007

    • Author(s)
      Senda M, Ito A, Tsuchida A, Hagiwara T, Kaneda T, Nakamura Y, Frukawa K
    • Journal Title

      Biochem J 402(3)

      Pages: 459-470

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and expression of a sialyltransferase responsible for the synthesis of disialylglactosylgloboside in normal and malignant kidney cells : downregulation of ST6GalNAc VI in renal cancers.2007

    • Author(s)
      Senda M, Ito A, Tsuchida A, Hagiwara T, Kaneda T, Nakamura Y, Frukawa K
    • Journal Title

      Biochem J. 402・3

      Pages: 459-470

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 腎細胞癌の現状と治療戦略.2006

    • Author(s)
      荒井陽一, 庵谷尚正, 伊藤明宏, 川村貞文, 佐藤信
    • Journal Title

      Pharma Medica 24・11

      Pages: 80-89

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Current state and treatment strategy of renal cell carcinoma2006

    • Author(s)
      Arai Y, Hiroya N, Ito A, Kawamura S, Sato M
    • Journal Title

      Pharma Medica 24・11

      Pages: 80-89

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 腎細胞癌の現状と治療戦略.2006

    • Author(s)
      荒井陽一, 庵谷尚正, 伊藤明宏, 川村貞文, 佐藤信.
    • Journal Title

      Pharma Medica 24・11

      Pages: 80-89

    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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