• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of gene expression on human viral nephropathy after renal transplantation

Research Project

Project/Area Number 17591696
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionOsaka City Univetsity

Principal Investigator

UCHIDA Junji  Osaka city university, graduate school of medicine, lecturer, 大学院医学研究科, 講師 (40343412)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordscytomegaloviral nephropathy / cDNA microarray / DNA tip / Renal transplantation / Chronic allograft nephropathy / renal transplantation / BK virus / cDNA microarray / cytomegalovirus / chronicallograft nephropathy / laser-capture microdissection
Research Abstract

To diagnose acute rejection induced chronic allograft nephropathy in the future and to judge the efficacy of treatment for rejection is often difficult. It is expected to detect the specific marker for acute rejection. We used DNA microarrays in a systematic study of gene-expression profile in tubulointerstitunum obtained by laser-captured microdissection in frozen biopsy samples from patients with acute rejection.
Biopsy samples from patients with acute rejection (n=3) and control (n=3) were embedded in Tissue Tek OCT medium and snap-frozen. The cryostat section (8 u m) were made, and tubulointestinum was laser microdissected with LM-2, Arcturus Engineering. Total RNA was extracted from dissected tissues. RNA was amplified by a T7 RNA polymerase-mediated RNA amplification reaction combined with an adaptor ligation-mediated PCR (TALPAT method). After biotinylated cRNAs were fragmented, they were hybridized to the DNA tip (Human genome U133 Plus 2.0 array: 54675 genes) and signal intensity was measured by Affymetrix gene Tip system. Data analysis was performed with Gene Spring. After eliminating data showing mismatch hybridization with negligible expression in both acute rejection and control group, 18356 genes were detected. Two hundred fifty three genes were twice or more differences in expression between acute rejection group and control group. We exploited gene tip technology to identify genes expression in the specific region where acute rejection occurred by biopsy samples from patients with acute rejection.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (15 results)

All 2006 2005

All Journal Article (15 results)

  • [Journal Article] Modified extravesical ureteroneocystostomy for completely duplicated ureters in renal transplantation.2006

    • Author(s)
      Uchida J, Naganuma T, Machida Y, Kitamoto K, Yamazaki T, Iwai T, Nakatani T.
    • Journal Title

      Urol Int. 77・2

      Pages: 104-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Molecular mechanisms and therapeutic strategies of chronic renal injury : the role of nuclear factor kappaB activation in the development of renal fibrosis.2006

    • Author(s)
      Tamoda S, Asai T, Kuwabara N, Iwai T, Uchida J, Teramoto K, Kaneda N, Yukimura T, Komiya T, Nakatani T, Miura K.
    • Journal Title

      J Pharmacol Sci. 100・1

      Pages: 17-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 腎血管疾患2006

    • Author(s)
      内田潤次
    • Journal Title

      腎と透析 61・増刊

      Pages: 426-429

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 免疫抑制薬はどこまで使い続けるか2006

    • Author(s)
      内田潤次, 長沼俊秀, 仲谷達也
    • Journal Title

      透析フロンティア 16・1

      Pages: 18-19

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Modified extravesical ureteroneocystostomy for completely duplicated ureters in renal transplantation.2006

    • Author(s)
      Uchida J, Naganuma T, Machida Y, Kitamoto K, Yamazaki T, Iwai T, Nakatani T.
    • Journal Title

      Urol Int 77-2

      Pages: 104-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular mechanisms and therapeutic strategies of chronic renal injury : the role of nuclear factor kappaB activati on in the development of renal fibrosis.2006

    • Author(s)
      Tamada S, Asai T, Kuwabara N, Iwai T, Uchida J, Teramoto K, Kaneda N, Yukimura T, Komiya T, Nakatani T, Miura K.
    • Journal Title

      J Pharmacol Sci 100-1

      Pages: 17-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An emulgent disease2006

    • Author(s)
      Uchida J
    • Journal Title

      A Kidney and dialysis,61・An extra number

      Pages: 426-429

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Where do you continue using an immunosuppressant to?2006

    • Author(s)
      Uchida J, Naganuma T, Nakatani T.
    • Journal Title

      A dialysis frontier 16-1

      Pages: 18-19

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular mechanisms and therapeutic strategies of chronic renal injury : the role of nuclear factor kappa B activation in the development of renal fibrosis.2006

    • Author(s)
      Tamada S, Asai T, Kuwabara N, Iwai T, Uchida J, Teramoto K, Kaneda N, Yukimura T, Komiya T, Nakatani T, Miura K.
    • Journal Title

      J Pharmacol Sci. 100・1

      Pages: 17-21

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 血液透析患者におけるPADAMの検討2005

    • Author(s)
      鞍作克之, 町田裕一, 岩井友明, 内田潤次, 土田健司, 杉村一誠, 吉村力勇, 仲谷達也
    • Journal Title

      大阪透析研究会会誌 23・1

      Pages: 35-39

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 診断に苦慮した、生体腎移植後促進型拒絶反応の1例2005

    • Author(s)
      石井啓一, 上川禎則, 杉本俊門, 京 昌弘, 山越恭雄, 長沼俊秀, 内田潤次, 仲谷達也
    • Journal Title

      大阪透析研究会会誌 23・1

      Pages: 41-44

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 生体腎移植希望患者に対する説明 -インフォームド コンセントの実際2005

    • Author(s)
      内田潤次, 長沼俊秀, 山崎健史, 町田裕一, 岩井友明, 桑原伸介, 杉村一誠, 仲谷達也
    • Journal Title

      大阪透析研究会会誌 23・1

      Pages: 51-54

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 両側Axillofemoral bypass留置患者に対する献腎移植の1例2005

    • Author(s)
      山崎健史, 石井淳一, 野村広徳, 岩井友明, 松村健太郎, 長沼俊秀, 鞍作克之, 内田潤次
    • Journal Title

      THERAPEUTIC RESEARCH 26・4

      Pages: 597-601

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 副腎腫瘍の一例2005

    • Author(s)
      杉村一誠, 内田潤次, 成田敬介, 岩井友明, 高原由姫, 仲谷達也, 長沼俊秀
    • Journal Title

      第16回大阪泌尿器画像診断研究会会誌 4

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Silent cerebral infarction predicts vascular events in hemodialysis patients.2005

    • Author(s)
      Uchida J, Tsuchida K, Takemoto Y, Tatsumi S, Sugimura K, Nakatani T
    • Journal Title

      Kidney International 67

      Pages: 2434-2439

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi