Investigation of progression and chemo-resistance associated, with loss of mitochondrial expression in urothelial carcinoma of the urinary bladder.
| Project/Area Number |
17591698
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kitasato University |
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Principal Investigator |
MATSUMOTO Kazumasa Kitasato University, School of Medicine, Lecturer (70306603)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Tetsuo Kitasato University, School of Medicine, Lecturer (00306599)
SATOH Takefumi Kitasato University, School of Medicine, Lecturer (50286332)
BABA Shiro Kitasato University, School of Medicine, Professor (00051889)
入江 啓 北里大学, 医学部, 講師 (50193694)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | mitochondria / bladder cancer / invasion / 薬剤耐性 / 呼吸能 / アポトーシス |
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| Research Abstract |
We investigated the association between mitochondrial expression and biology of bladder cancer using established cell lines. Initially, we constructed the bladder cancer cell line without all mitochondria in the nucleus made from RT4 parent cell and named as RT4 ρ0. RT4 showed the papillary shape and aggregated growth in vitro. However, RT4ρ0 had the morphological changes like invasive cancer in terms of T24 which was representative of high grade bladder cancer cell line, and showed diffused growth. RT4ρ0 was significantly increased the cell numbers compared to RT4 during the same incubation time.
In addition, RT4ρ0 acquired the higher resistant for the chemotherapy agent, cisplatin, than RT4, but not significant. In addition, we also investigated the differences of DNA and protein expressions between RT4 ρ 0 and RT4. There were quit different expressions of DNA using DNA microarray. RT4 ρ 0 had approximately fifty thousand different spots compared with RT4. RT4 ρ 0 had also quit different expressions of its biological proteins including apoptotic and oxidation reduced proteins determined by two dimensional electrophoresis. Interestingly, we found several biologically and chemically unknown proteins in this experiment. According to these results, we demonstrated an association of a role of mitochondria with bladder cancer biology including a growth activity and an acquisition of chemo-resistance. Further experiments were warranted for the next step in regard to show the mechanism of these DNA hot spots and unknown proteins.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Expression of S100A2 and S100A4 predicts disease progression and patient survival in bladder cancer.2007
Author(s)
Matsumoto, K, Irie, A, Satoh, T, Ishii, J, Iwabuchi, K, Iwamura, M, Egawa, S, Baba, S
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Journal Title
Urology 70(3)
Pages: 602-607
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Gemcitabine and paclitaxel chemotherapy as a second-line treatement for advanced transitional cell carcinoma2007
Author(s)
Matsumoto, K, Irie, A, Satoh, T, Okazaki, M, Iwamura, M, Baba, S
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Journal Title
Int J Urol 14
Pages: 1000-1004
Description
「研究成果報告書概要(欧文)」より
Related Report
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