Project/Area Number |
17591700
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Urology
|
Research Institution | Kyorin University |
Principal Investigator |
OKEGAWA Takatsugu Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (70306679)
|
Co-Investigator(Kenkyū-buntansha) |
NUTAHARA Kikuo Kyorin University, School of Medicine, Professor, 医学部, 教授 (00143470)
HIGASHIHARA Eiji Kyorin University, School of Medicine, Professor, 医学部, 教授 (00092312)
渡辺 和吉 杏林大学, 医学部, 助手 (60407054)
|
Project Period (FY) |
2005 – 2006
|
Project Status |
Completed (Fiscal Year 2006)
|
Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
Keywords | bladder cancer / hypermethylation / FANCF gene |
Research Abstract |
PURPOSE : We investigate the extent of promoter hypermethylation in TCC throughout the urinary tract. PATIENTS AND METHODS : Tissue was obtained from 120 patients (median followup, 38 months) with urothelial cancer. Analysis for methylation at FANCF, using methylation-sensitive polymerase chain reaction and bisulfite sequencing, was performed for each sample and compared with the tumor's clinicopathologic details, microsatellite instability status, and subsequent behavior. RESULTS : Promoter methylation was present in 86% of urothelial cancer. In grade, methylation at FANCF indicated grade1 : 15%, grade2 : 28%, grade3 : 68%. In stage, methylation at FANCF indicated pTa : 12% pT1: 34%, pT2-4 : 57%. Methylation of FANCF was associated with advanced tumor stage (P =.0002) and higher tumor progression (P = 0.04. CONCLUSION : Methylation occurs commonly in urinary tract tumors, may affect carcinogenic mechanisms, and is a prognostic marker and a potential therapeutic target.
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