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The relation with cytoskeletal changes of human ooyte and their age.

Research Project

Project/Area Number 17591711
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

NAKAMURA Soichi  TOHOKU UNIVERSITY, HOSPITAL, RESEARCH ASSOCIATE, 病院, 助手 (00343054)

Co-Investigator(Kenkyū-buntansha) TERADA Yukihiro  TOHOKU UNIVERSITY, HOSPITAL, ASSOCIATE PROFESSOR, 病院・助教授 (10260431)
MURAKAMI Takashi  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院医学研究科, 助教授 (20240666)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsaging / oocyte / cytoskeleton / in vitro maturation / fertilization / 細胞内小器官 / 微小管形成中心
Research Abstract

We reported about cytoskeletal changes oocyte and sperm in fertilization. In current study, we tried to take human immature oocytes from patients who underwent gynecological surgery and these oocytes cultured in 37℃, 5%CO2 atmosphere at 24hours to maturate. The culture medium was made from P-1 medium with a little changes given reported by Cekeleniak in 2001. The oocytes were collected from three polycystic ovarian syndrome patients who underwent laparoscopic ovarian dolling. The first case is 25 years old and eight immature oocytes were collected. After culture for maturation at 24 hours, these oocytes changed into six metaphase II (MII) eggs, one germinal vesicle (GV) egg and one degenerate egg. The second case was 26 years old and twelve oocytes were collected. These oocytes changed into two MII eggs, four GV eggs and six degenerate eggs. The third case was 33 years old and four oocytes were collected. These oocytes changed into one MII egg, two GV eggs and one degenerate egg. The rate of successes of maturation culture was 37.5%. These eggs were fixed and observed alpha-beta tubuline and DNA by immunohistochemical method that reported S Simerly and G Schatten. We recognized these spindles were no specific differences from in vitro maturated oocyte in tubuline and chromosome structure to in vivo matured eggs. We tried to observe the real time changes of spindles of culture eggs by poloscope. However, all of eggs that observed by poloscope were degenerated though we could not get any specific changes in this experiment by poloscope. In conclusion, we studied in vitro culture for human immature oocytes into mature eggs, there were no changes in cytoskeletal observation. This culture method will useful for research about human oocyte maturation kinetics. Further, We will study the in vitro culture system to another age of people.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (24 results)

All 2007 2006 2005

All Journal Article (24 results)

  • [Journal Article] Positive expression of the immunoglobulin superfamily protein IZUMO on human sperm of severe male infertile patients2007

    • Author(s)
      Hayasaka S, et al.
    • Journal Title

      Fertility and Sterility. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of photodynamic therapy using 5-aminolevulinic acid on cultured human adenomyosis-derived cells.2007

    • Author(s)
      Suzuki-Kakisaka Haruka, et al.
    • Journal Title

      Fertility and Sterility. 87

      Pages: 34-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] POSITIVEEXPRESSION OF THE IMMUNOGLOBULIN SUPERFAMILY PROTEIN IZUMO ON HUMAN SPERM OF SEVERE MALE INFERTILE PATIENTS2007

    • Author(s)
      HAYASAKA SINICHI
    • Journal Title

      FERTIL STERIL (IN PRESS)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] EFFECTS OF PHOTODYNAMIC THERAPY USING 5-AMINOLEVULINIC ACID ON CULTURED HUMAN ADENOMYOSIS-DERIVED CELLS.2007

    • Author(s)
      SUZUKI-KAKISAKA HARUKA
    • Journal Title

      FERTIL STERIL 87

      Pages: 34-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional analysis of the sperm centrosome- inplications for assisted reproductive technique.2007

    • Author(s)
      Yukihiro Terada
    • Journal Title

      In Gamate biology. 1st edition

      Pages: 507-515

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Functional analyses of the sperm cetrosome in human reproduction.2006

    • Author(s)
      Terada Yukihiro
    • Journal Title

      Curr Top Dev Biol. 63

      Pages: 507-507

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A selective cyclooxynase (COX)-2 inhibitor suppresson the growth of endometriosis xenografts via anti-angiogenetic activity in severe combined immunodeficiency mice.2006

    • Author(s)
      Ozawa Yuka, et al.
    • Journal Title

      Fertility and Sterility. 86

      Pages: 1146-1151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cytoskeletal Dynamics Post-ICSI During fertilization.2006

    • Author(s)
      Hayasaka Sinichi, et al.
    • Journal Title

      J Mamm Ova Res. 23

      Pages: 21-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pregnancy in a patient with retoroperitoneal fibrosis who underwent in vitro fertilization.2006

    • Author(s)
      Nakamura Soichi, et al.
    • Journal Title

      Nephrol Dial Transplant. 21

      Pages: 2669-2669

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sccessful strategy for the hysteroscopic myomectomy of a submucous myomaarising from the fundus.2006

    • Author(s)
      Murakami Takasi
    • Journal Title

      Fertility and Sterility. 86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FUNCTIONAL ANALYSES OF THE SPERM CENTROSOME IN HUMAN REPRODUCTION : IMPLICATIONS FOR ASSISTED REPRODUCTIVE TECHNIQUE.2006

    • Author(s)
      TERADA YUKIHIRO
    • Journal Title

      CURR TOP DEV BIOL 507

      Pages: 63-63

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A SELECTIVE CYCLOOXNASE(COX)-2 INHIBITOR SUPPRESSION THE GROWTH OF ENDOMETRIOSIS XENOGRAFTS VIA ANTI-ANGIOGENETIC ACTIVITY IN SEVERE COMBINED IMMUNODEFICIENCY MICE.2006

    • Author(s)
      OZAWA YUKA
    • Journal Title

      FERTIL STERIL 86

      Pages: 1146-1151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CYTOSKELETAL DYNAMICS POST-ICSI DURING FERTILIZATION.2006

    • Author(s)
      HAYASAKA SHINICHI
    • Journal Title

      J MAMM OVA RES 23

      Pages: 21-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] PREGNANCY IN A PATIENT WITH RETROPERITONEAL FIBROSIS WHO UNDERWENT IN VITRO FERTILIZATION.2006

    • Author(s)
      NAKAMURA SOICHI
    • Journal Title

      NEPHROL DIAL TRANSPLANT 21

      Pages: 2669-2669

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SUCCESSFUL STRATEGY FOR THE HYSTEROSCOPIC MYOMECTOMY OF A SUBMUCUCOS MYOMA ARISING FROM THE FUNDUS2006

    • Author(s)
      TAKASHI MURAKAMI
    • Journal Title

      FERTIL STERIL 86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 精子中心体機能と受精障害2006

    • Author(s)
      中村 聡一
    • Journal Title

      産婦人科の実際 55-2

      Pages: 185-195

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 精子の受精能2006

    • Author(s)
      寺田 幸弘
    • Journal Title

      産科と婦人科 73-6

      Pages: 727-733

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Post ICSI events; choreography of fertilization.2006

    • Author(s)
      Yukihiro Terada
    • Journal Title

      Journal of mammalian ova, research 23-1

      Pages: 21-26

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A Trial to Restore Defective Human Sperm Centrosomal Function2005

    • Author(s)
      Nakamura S, Terada Y, Murakami T et al.
    • Journal Title

      Human Reproduction 20

      Pages: 1933-1937

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dynamics of Microtubules and Positioning of Female Pronucleus during Bovine Parthenogenesis.2005

    • Author(s)
      Morito Y, Terada Y, Nakamura S et al.
    • Journal Title

      Biology of Reproduction 73

      Pages: 935-941

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome2005

    • Author(s)
      Morita J, Terada Y, Nakamura S et al.
    • Journal Title

      Reproductive Medicine and Biology 4

      Pages: 169-177

    • NAID

      10015654878

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cytoskeletal Dynamics During Mammalian Gametegenesis and Fertilization : Implication for Human Reproduction2005

    • Author(s)
      Terada Y, Nakamura S, Muarakami T et al.
    • Journal Title

      Reproductive Medicine and Biology 4

      Pages: 179-187

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sequential Changes in The Cytoskeletal Dynamics of Microtubules and Microfilaments : Morphological analysis in human spermiogenesis2005

    • Author(s)
      Tachibana M, Terada Y, Murakami T, et al.
    • Journal Title

      Fertility and Sterility 84

      Pages: 1241-1284

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Reproduction of menstrual changes in transplanted human endometrial tissue in immunodeficient mice.2005

    • Author(s)
      Matuura Sawada R, Murakami T, Terada Y, et al.
    • Journal Title

      Human Reproduction 20

      Pages: 1477-1484

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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