| Project/Area Number |
17591714
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamagata University |
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Principal Investigator |
TAKAHASHI Toshifumi (2007) Yamagata University, School of Medicine, Associate professor (20302292)
手塚 尚広 (2005-2006) 山形大学, 医学部, 助教授 (60261690)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUMI Seiji Yamagata University, School of Medicine, Associate professor (50323168)
KURACHI Hirohisa Yamagata University, School of Medicine, Professor (40153366)
高橋 俊文 山形大学, 医学部, 助手 (20302292)
五十嵐 秀樹 山形大学, 医学部, 助手 (80333970)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Ion channels / Smooth muscle cells / Pregnancy / Calcium channel / Potasium channel / 分子生理学 |
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| Research Abstract |
This is the last year of this study. So we analyzed the results taken from the experiments described below. First, we performed the experiment of expression of ion channels in pregnant smooth muscle cells; Expression of calcium channels, such as inositol tri-phosphate (IP3) receptors and ryanodine receptors; Expression of sodium and potassium channels. Expression of calcium channels, such as IN and ryanodine receptors in rat myometrium were increased with increasing gestational age. Second, we performed the experiment of the effects of oxytocin and beta-stimulant on pregnant myometrium by using electrophysiological approach. Third, we performed the experiment of the effects of oxytocin and beta-stimulants on intracellular calcium concentrations in pregnant myometrium. The intracellular calcium concentration in pregnant myometrium was increased dose-dependently by treatment of oxytocin. The increased calcium concentration induced by oxytocin was inhibited by treatment of beta-stimulants. These results suggest that the expression and function of various ion channels in smooth muscle cells are modulated by gestational age.
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