Functional analysis of cholesterol sulfate in interaction between embryo and endometrium.
| Project/Area Number |
17591717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MOMOEDA Mikio The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (50221627)
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| Co-Investigator(Kenkyū-buntansha) |
HIROI Hisahiko The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部附属病院, 助手 (10343138)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | cell and tissue / embryogenesis / implantation / endometrium / gene |
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| Research Abstract |
We have studied on a tempo-spatial control of embryo implantation to uterine endometrium, demonstrating that the content of cholesterol sulfate(CS) in endometrium increases transiently during implantation period, the increase of CS is observed only in peri-implantation region, and CS inhibits the invasion of trophoblast to endometrium. In this study, we aimed to elucidate the function of CS synthesis in interaction between embryo and endometrium during implantation.
1.It became clear that CS inhibit MMP activity as well as plasmin activity in the culture medium of endometrial cell culture system. We suggest that CS plays an important role in regulating a protease activity in vivo during implantation.
2.CS promoted gene expression of RevErb regarded as a target gene of ROR in an endometrial cell. In addition, CS promoted gene expression of ROR which was the receptor of CS.
3.In JEG which was cell line of trophoblast, CS stimulated a progesterone production. We reviewed action of CS on gene expression of enzymes associated with a progesterone production, suggesting no effect on them. Now we are examining the affinity of CS for cholesterol transportation protein.
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Report
(3 results)
Research Products
(3 results)
