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Heparanase expression correlates with angiogenesis and predicts tumor metastasis in endometrial cancer.

Research Project

Project/Area Number 17591723
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionUniversity of the Ryukyus (2006)
Niigata University (2005)

Principal Investigator

AOKI Yoichi  Univ. of the Ryukyus, Faculty of Med., Dept Ob/Gyn, Professor, 医学部, 教授 (40231774)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsheparanase / RT-PCR / anti-heparanase antibody / endometrial cancer / angiogenesis / imunohistochemical staining / 婦人科癌 / 転移 / 予後因子 / 転移抑制
Research Abstract

The human heparanase has been shown to function in tumor progression, metastatic spread, and tumor angiogenesis. The aim of the present study was to assess heparanase expression in endometrial cancer in correlation with neovascularization and clinicopathological factors. Fifty-two endometrial cancers were obtained from previously untreated patients (median age, 56 years, range, 35-80 years). The expression of heparanase mRNA was evaluated using a semi-quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical staining (IHC) with anti-heparanase polyclonal antibody. This antibody was raised by immunizing a rabbit with a peptide containing the amino acid residues from 238 to 250 of the Heparanase. Tumor angiogenesis was assessed using microvessel counting. The Mann-Whitney U test, one factor ANOVA test, and Spearman' s test were used to determine the relationship between heparanase expression, microvessel density, and clinicopathological parameters. The expression of heparanase mRNA was detected in 26 of 52 (50%) endometrial cancers, and was significantly correlated with FIGO stage IIIc (p=0.0075), the presence of lymph-vascular space involvement (LVSI) (p=0.0041), lymph node metastasis (LNM) (p=0.0049), and histological tumor grade (p=0. 003). IHC showed that the heparanase was expressed in 23 of the 52 (44. 2%) samples, which was significantly related to LVSI (p=0.0028), depth of myometrial invasion (p=0.0026), and histological tumor grade (p=0.0135). Microvessel density was also associated with FIGO stage IIIc (p=0.027), LVSI (p=0.001), LNM (p=0.038), ovarian metastasis (p=0.03) and histological tumor grade (p=0.003). Moreover, we found a strong positive correlation between heparanase expression and microvessel density (r2=0.475, p=0.0001). These results suggest that the expression of heparanase can promote tumor angiogenesis and develop metastasis in endometrial cancer.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (24 results)

All 2006 2005

All Journal Article (21 results) Book (3 results)

  • [Journal Article] Tumor lysis syndrome associated with weekly paclitaxel treatment in a case with ovarian cancer.2006

    • Author(s)
      Yahata T
    • Journal Title

      Gynecol Oncol 103

      Pages: 752-754

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Metastatic choriocarcinoma successfully treated with paclitaxel and carboplatin after interstitial lung disease induced by EMA-CO.2006

    • Author(s)
      Amikura T
    • Journal Title

      Gynecol Oncol 102

      Pages: 573-575

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Retrieval of intraperitoneal Penrose drain under transvaginal endoscopic guidance.2006

    • Author(s)
      Takeyama S
    • Journal Title

      Gynecol Oncol 102

      Pages: 391-393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Long-term conservative therapy for endometrial adenocarcinoma in young women.2006

    • Author(s)
      Yahata T
    • Journal Title

      Hum Reprod 21

      Pages: 1070-1075

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prediction of myometrial invasion in patients with endometrial carcinoma : Comparison of magnetic resonance imaging, Transvaginal ultrasonography, and gross visual inspection2006

    • Author(s)
      Yahata Y
    • Journal Title

      Eur J Gynaecol Oncol 15

      Pages: 1199-1202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Retrieval of intraperitoneal Penrose drain under transvaginal endoscopic guidance2006

    • Author(s)
      Takeyama S
    • Journal Title

      Gynecol Oncol 102

      Pages: 391-393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prediction of myometrial invasion in patients with endometrial carcinoma : Comparison of magnetic resonance imaging, Transvaginal ultrasonography, and gross visual inspection.2006

    • Author(s)
      Yahata Y
    • Journal Title

      Eur J Gynaecol Oncol 15

      Pages: 1199-1202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of Immunohistochemical Expression of BRCA1 in Ovarian Carcinoma.2006

    • Author(s)
      Aoki Y
    • Journal Title

      HANDBOOKS of IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION OF HUMAN CARCINOMAS (Elsevier Academic Press, Burlington, MA, USA)

      Pages: 339-374

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tumor lysis syndrome associated with weekly paclitaxel treatment in a case with ovarian cancer.2006

    • Author(s)
      Yahata T, NIshikawa N, Aoki Y, Tanaka K
    • Journal Title

      Gynecol Oncol 103

      Pages: 752-754

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Metastatic choriocarcinoma successfully treated with paclitaxel and carboplatin after interstitial lung desease induced by EMA-CO.2006

    • Author(s)
      Amikura T, Aoki Y, Banzai C, Yokoo T, Nishikawa N, Sekine M, Suzuki M, Tanaka K
    • Journal Title

      Gynecol Oncol 102

      Pages: 573-575

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Retrieval of intraperitoneal Penrose drain under transvaginal endoscopic guidance.2006

    • Author(s)
      Takeyama S, Aoki Y, Kamimura N, Suzuki M, Tanaka K
    • Journal Title

      Gynecol Oncol 102

      Pages: 391-393

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Long-term conservative therapy for endometrial adenocarcinoma in young women.2006

    • Author(s)
      Yahata T, Fujita K, Aoki Y, Tanaka K
    • Journal Title

      Hum Reprod 21

      Pages: 1070-1075

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prediction of myometrial invasion in patients with endometrial carcinoma : Comparison of magnetic resonance imaging, Transvaginal ultrasonography, and gross visual inspection.2006

    • Author(s)
      Yahata Y, Aoki Y, Tanaka K
    • Journal Title

      Eur J Gynaecol Oncol 15

      Pages: 1199-1202

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Yolk sac tumor of the ovary during pregnancy2005

    • Author(s)
      Aoki Y, Higashino M, Ishii S, Tanaka K
    • Journal Title

      Gynecologic Oncolgy 99

      Pages: 497-499

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Irinotecan hydrochloride (CPT-11) andn mitomycin C as the first ine chemotherapy for ovarian clear cell adnocarcinoma2005

    • Author(s)
      Nishino K, Aoki Y, Tanaka K et al.
    • Journal Title

      Gynecologic Oncology 97

      Pages: 893-897

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stage I squamous cell carcinoma of vagina complicating pregnancy ; Successful conservative treatment2005

    • Author(s)
      Fujita K, Aoki Y, Tanaka K
    • Journal Title

      Gynecologic Oncology 98

      Pages: 513-515

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Placental site trophoblastic tumor presenting as subaponeurotic metastasis2005

    • Author(s)
      Aoki Y, Kase H, TanakaK et al.
    • Journal Title

      International Journal of Gynecological Cancer 15

      Pages: 385-388

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Complete response to Docetaxel and Carboplatin chemotherapy for a stage IV uterine papillary serous carcinoma2005

    • Author(s)
      Kashima K, Aoki Y, Tanaka K et al.
    • Journal Title

      International Journal of Gynecological Cancer 15

      Pages: 1199-1202

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Irinotecan hydrochloride (CPT-11) and mitomycin C in docetaxel-carboplatin (DJ) refractory ovarian cancer2005

    • Author(s)
      Aoki Y, Amikura T, Tanaka K et al.
    • Journal Title

      Proc Am Soc Clin Oncol 23

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Increased efficiency of cisplatin resistant cell lines to DNA mediated gene transfer with cationic liposome2005

    • Author(s)
      Sato T, Aoki Y, Tanaka K et al.
    • Journal Title

      Journal of Obstetrics and Gynaecology Research 31

      Pages: 368-374

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Meetastatic ovarian tumor 14 years after initial surgery for a gastric cancer2005

    • Author(s)
      Kurata H, Aoki Y, Tanaka K et al.
    • Journal Title

      Journal of Obstetrics and Gynecology 25

      Pages: 321-322

    • Related Report
      2005 Annual Research Report
  • [Book] HANDBOOKS of IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION OF HUMAN CARCINOMAS2006

    • Author(s)
      Aoki Y
    • Total Pages
      564
    • Publisher
      Elsevier Academic Press, Burlington, MA, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] HANDBOOKS of IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION OF HUMAN CARCINOMAS2006

    • Author(s)
      Aoki Y, Tanaka K
    • Total Pages
      564
    • Publisher
      Elsevier Academic Press, Burlington, MA, USA
    • Related Report
      2006 Annual Research Report
  • [Book] Progress in Ovarian Cancer ; Docetaxel in Combination with Carboplatin as first-line chemotherapy for Patients with Epithelial Ovarian Cancer2005

    • Author(s)
      Aoki Y, Tanaka K
    • Total Pages
      323
    • Publisher
      Noca Science
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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