| Project/Area Number |
17591749
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
KAWAGOE Yasuyuki University of Miyazaki, Faculty of Medicine, Dept.Ob&Gy, Assistant Professor (70336311)
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| Co-Investigator(Kenkyū-buntansha) |
SAMESHIMA Hiroshi University of Miyazaki, Faculty of Medicine, Dept.Ob&Gy, Associate Professor (50274775)
IKENOUE Tsuyomu University of Miyazaki, Faculty of Medicine, Dept.Ob&Gy, Professor (60232211)
坂本 紘 鹿児島大学, 農学部, 教授 (20041650)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | intermittent umbilical cord occlusion / variable deceleration / non-acidemic hypoxemia / fetal brain damage / 変動制一過性徐脈 / アシドーソスを伴わない低酸素血症 / 低酸素症 / 新生児脳症 / 臍帯因子 |
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| Research Abstract |
Objectives : Neurologic deficit in children can be attributed to prenatal events and asphyxial insults. In human pregnancies near term, intermittent 'variable type' fetal heart rate (FHR) decelerations are seen in 5% and can be associated with umbilical cord occlusion (UCO). Effect of intermittent UCO on neurohistology in fetus is determined. Clinical detection for hypo-perfusion of the fetal brain is also investigated
Methods : 14 chronically instrumented fetal sheep (experimental, n=7, Control, n=7) at 0.8 gestation were studied over 4 days with UCOs by complete inflation of an occluder cuff (duration 90 seconds) every 30 minutes for 3 to 5 hours each day. Animals were then euthanized and the fetal brains were prepared for subsequent histology and immunostaining for myelin(SCR), astrocytes(GFAP), and activated microglia(HLADR). Brain injury was scored by a neuropathologist, based on the severity of injury by histopathology and changes in immunostaining ; 0-normal, 1-minimal, 2-mild/ea
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rly, 3-moderate to severe/advanced. We also studied the circulatory change by using blood pressure and SpO_2 charges in goat fetus. These experiments were conducted in the Department of Veterinary Medicine of Kagoshima University and Department of Obstetrics and Gynecology of Western Ontario University, Canada.
Results : Fetal PO_2 decreased from 24.0±0.5 to 6.7±0.8mmHg whereas change of PH was from 7.36 to 7.31. All the experiment group showed minimal injury (increased staining of GFR and/or HLADR) or mild injury (plus in ischemic neurons in cortex). Brain injury score for controls was significantly lower, averaging 0.4±0.25(p<0.01) with one animals having minimal injury and 1.6±0.20 in experimental group.
Conclusions : We conclude that intermittent UCO can result in neuropathological change in the cortical and subcortical white and gray matters. We speculate that intermittent UCO may give rise to postnatal neurologic deficit. New technique to detect brain hypo-perfusion is required in clinical settings. Less
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