| Project/Area Number |
17591751
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
UMESAKI Naohiko Wakayama Medical University, School of Medicine, Professor, 医学部, 教授 (20106339)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Tetsuji Wakayama Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (80275255)
YUKAWA Kazunori Wakayama Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20301434)
UTSUNOMIYA Hirotoshi Wakayama Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (60264876)
岩橋 正明 和歌山県立医科大学, 医学部, 講師 (90203398)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
|
|---|
| Keywords | endometrium / embryo implantation / cytokine / endometriosis / regeneration medicine |
|---|
| Research Abstract |
For mouse hepatitis virus pollution of university animal center in spring of 2006, all our mice were killed and the animal experiments were ceased. Since virus infection was re-appeared half a year after disinfection, however, the animal experiment center has been closed yet. We, therefore, had to switch plans of this study.
(1) Analysis of mouse endometrial remodeling process and identification of murine endometrial stem cells:
Short-term endometrial rebuilding was succeeded in by mixed culture transplant of plural endometrial cell strains, but animal experiment is stopped by the above reason, and long-term observation cannot be finished.
(2) Analysis of human endometrial remodeling process and identification of human endometrial stem cells:
<1> Cellular characterization of human endometrial stromal cells
(i) Influence of various bioactive substances: Influence such as various cytokines, bioactive substances, cell death inducers such as anticancer drugs, siRNA experiments were examined (pr
… More
eparation in submission). Involvement of TRAIL and TRAIL receptor was made clear by DAPK knockdown experiments (submitted).
(ii) Apoptosis sensitivity: Endometrial stem-like cells had resistance to various apoptotic signals such as anticancer drugs and Fas stimulants.
(iii) Differentiation activity: Partial differentiation was confirmed by mixed culture experiments.
<2> Establishment of in vitro differentiation of human endometrial stem-like cells to make differentiation pedigree of human endometrial cells
Partial differentiation phenomenon was confirmed by mixed culture experiments, but the experiments are ongoing now. Establishment of endometrial reproduction medical treatment by mouse intrauterine transplantation of two human endometrial stem-like cells. Animal experiments have been stopped because of the above reasons. Three human endometrial stem-like cell-specific antigen were identified. In order to establish hybridomas producing anti-endometrial stem cell monoclonal antibodies balb/c mice were immunized with human endometrial stem-like cells. However, immunized mice were killed due to contamination of the animal center. When the animal experiments were allowed, the plan will be re-started. Less
|
