| Project/Area Number |
17591772
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NAKAMARU Yuji Hokkaido Univ., Hokkaido University Hospital, Inst., 病院, 助手 (20344509)
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| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Macrophage migration inhibitory factor / MIF / allergic rhinitis / cytokine / アレルギー性鼻炎 |
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| Research Abstract |
To elucidate Macrophage migration inhibitory factor (MIF)'s role in the pathogenesis of allergic rhinitis (AR), we sensitized MIF-deficient gene knockout (KO) mice and wild type (WT) mice intraperitoneally with ovalbumin (OVA) and compared their clinical symptoms and allergic responses after intranasal challenge. Antigen-induced nasal symptoms were significantly reduced in MIF KO mice compared to WT mice. Histological examination of nasal mucosa showed that the number of infiltrating eosinophils in MIF KO mice was significantly lower than that in WT mice (P< 0.05). The concentration of TNF-α in nasal mucosa was also significantly lower in MIF KO mice than in WT mice (P < 0.05). We have demonstrated that the absence of MIF affects several aspects of experimental AR. One mechanism by which these effects might be mediated is by down regulating TNF-α. The block of allergic inflammation in MIF KO mice suggests MIF has an extremely important role in the allergic response.
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