| Project/Area Number |
17591774
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Tohoku University |
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Principal Investigator |
YOSHIDA Naohiro Tohoku University, GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院医学系研究科, 非常勤講師 (90291260)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Hiroshi Tohoku University, GRADUATE SCHOOL OF ENGINEERING, PROFESSOR, 大学院工学研究科, 教授 (30111264)
菊地 俊彦 東北大学, 大学院・医学系研究科, 助教授 (70177799)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | acoustic trauma / heat stress / hair cell |
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| Research Abstract |
Overexposure to intense sound damages the inner ear cells can lead to permanent threshold shift (PTS). Conditioning effect can protect the ear from acoustic injury. The mechanism of this conditioning effect has been still unknown.
In this research project, the mechanism of conditioning effect has been clarified by using 1) stiffness measurement by atomic force microscopy, 2) histological findings of f-actin and prestin by confocal microscope, 3) electrophysiological assay by DPOAE measurement. CBA strain mice were used in this project. The stiffness of outer hair cells has been elevated at 3, 6 hours after heat stress (41.5 degree, 15min). Furthermore, f-actin also has been elevated for 12 hours after heat stress. On the other hand, prestin which is the specific motor protein in outer hair cells, has not been elevated after heat stress. The enhancement of DPOAEs input-output function were observed in the same time, 3 to 6 hours after heat stress. PTS was protected dramatically at 6hours heat stress-trauma interval. These results would show the mechanism of conditioning effect by changing the stiffness with f-actin in inner hair cells.
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