Gene expression assay using Microarray technique between embryonic stem cell and inner ear sphere formation cells.
| Project/Area Number |
17591785
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KOJIMA Ken Kyoto University, Graduate School of Medicine, Researcher of COE, 医学研究科, COE研究員 (60378685)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Akiko Kyoto University, Graduate School of Medicine, Clinical Fellow, 医学研究科, 医員 (30378731)
藤野 清大 京都大学, 医学研究科, 助手 (50359832)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | embryonic stem cell / inner ear progenitor cell / microarray / expression gene / cyclopedic analysis / transcription factor / ES細胞 / 内耳原器 / 発現遺伝子の網羅的解析 / 内耳発達 |
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| Research Abstract |
In the human inner ear, after loss of hair cell in the organ of Corti and spiral ganglion cells, the hair cells and ganglion cells do not regenerate spontaneously. Previously, it has been shown that cell transplantion to the damaged inner ear is a candidate therapy for permanent hearing loss. But, we have severe problems on securing of cell source for the cell transplantation. It was reported that embryonic stem cells possess potential to differentiate into hair cell phenotypes. The embryonic stem cells are possible candidate to overcome the problems. To develop methods of induction of differentiation of embryonic stem cell into the inner ear hair cells and spiral ganglion neurons, we need to know differences between the embryonic stem cells and the hair cells and ganglion neurons. In this study, we compared genes expressed in embryonic stem cells and cell cultures obtained from mouse developing inner ear. First, we established sphere formation cell cultures from embryonic day 10 mouse inner ears which containing immature inner ear cells. Second, expression genes of the embryonic stem cells and the otic sphere forming cell cultures were profiled using Agilent array, and then analyzed cyclopedically. The results of this assay showed that genes related in neural lineages, e. g. Olig1 and 01ig2, were expressed in otic shere forming cell cultures more predominantly than embryonic stem cell cultures. The profiles made in this study may lead us to develop new procedures to generate hair cells and ganglion neurons from embryonic stem cells. In this period, we contribute to researches of animal models of hearing loss and cell trasnplantation (Imura et al. Scinece 2007 in printing, Sekiya et al. Experimental Neurology 198: 12-24, 2005, Sekiya et. al. Eur J Neurol 25 : 2307-18, 2007).
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] alpha-Klotho asa a regulator of calcium homeostasis.2007
Author(s)
Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, Obuse C, Togashi K, Tominaga M, Kita N, Tomiyama K, Iijima J, Nabeshima Y, Fujioka M, Asato R, Tnanaka S, Kojima K, Ito J, Nozaki K, Hashimoto N, Ito T, Nishio T, Uchiyama T, Fujimori T, Nabeshima Y
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Journal Title
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Alpha-Klotho asa a regulator of calcium homeostasis.2007
Author(s)
Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, Obuse C, Togashi K, Tominaga M, Kita N, Tomiyama K, Iijima J, Nabeshima Y, Fujioka M, Asato R, Tnanaka S, Kojima K, Ito J, Nozaki K, Hashimoto N, Ito T, Nishio T, Uchiyama T, Fujimori T, Nabeshima Y.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] alpha-Klotho asa a regulator of calcium homeostasis.2007
Author(s)
Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, Obuse C, Togashi K, Tominaga M, Kita N, Tomiyama K, Iijima J, Nabeshima Y, Fujioka M, Asato R, Tnanaka S, Kojima K, Ito J, Nozaki K, Hashimoto N, Ito T, Nishio T, Uchiyama T, Fujimori T, Nabeshima Y
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Related Report
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