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Gene expression assay using Microarray technique between embryonic stem cell and inner ear sphere formation cells.

Research Project

Project/Area Number 17591785
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KOJIMA Ken  Kyoto University, Graduate School of Medicine, Researcher of COE, 医学研究科, COE研究員 (60378685)

Co-Investigator(Kenkyū-buntansha) NISHIDA Akiko  Kyoto University, Graduate School of Medicine, Clinical Fellow, 医学研究科, 医員 (30378731)
藤野 清大  京都大学, 医学研究科, 助手 (50359832)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsembryonic stem cell / inner ear progenitor cell / microarray / expression gene / cyclopedic analysis / transcription factor / ES細胞 / 内耳原器 / 発現遺伝子の網羅的解析 / 内耳発達
Research Abstract

In the human inner ear, after loss of hair cell in the organ of Corti and spiral ganglion cells, the hair cells and ganglion cells do not regenerate spontaneously. Previously, it has been shown that cell transplantion to the damaged inner ear is a candidate therapy for permanent hearing loss. But, we have severe problems on securing of cell source for the cell transplantation. It was reported that embryonic stem cells possess potential to differentiate into hair cell phenotypes. The embryonic stem cells are possible candidate to overcome the problems. To develop methods of induction of differentiation of embryonic stem cell into the inner ear hair cells and spiral ganglion neurons, we need to know differences between the embryonic stem cells and the hair cells and ganglion neurons. In this study, we compared genes expressed in embryonic stem cells and cell cultures obtained from mouse developing inner ear. First, we established sphere formation cell cultures from embryonic day 10 mouse inner ears which containing immature inner ear cells. Second, expression genes of the embryonic stem cells and the otic sphere forming cell cultures were profiled using Agilent array, and then analyzed cyclopedically. The results of this assay showed that genes related in neural lineages, e. g. Olig1 and 01ig2, were expressed in otic shere forming cell cultures more predominantly than embryonic stem cell cultures. The profiles made in this study may lead us to develop new procedures to generate hair cells and ganglion neurons from embryonic stem cells. In this period, we contribute to researches of animal models of hearing loss and cell trasnplantation (Imura et al. Scinece 2007 in printing, Sekiya et al. Experimental Neurology 198: 12-24, 2005, Sekiya et. al. Eur J Neurol 25 : 2307-18, 2007).

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (16 results) Book (2 results)

  • [Journal Article] alpha-Klotho asa a regulator of calcium homeostasis.2007

    • Author(s)
      Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, Obuse C, Togashi K, Tominaga M, Kita N, Tomiyama K, Iijima J, Nabeshima Y, Fujioka M, Asato R, Tnanaka S, Kojima K, Ito J, Nozaki K, Hashimoto N, Ito T, Nishio T, Uchiyama T, Fujimori T, Nabeshima Y
    • Journal Title

      Science (in printing)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve.2007

    • Author(s)
      Sekiya T, Holley MC, Kojima K, Matsumoto M, Helyer R, Ito J
    • Journal Title

      Eur J Neurosci 25(8)

      Pages: 2307-2318

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Rebuilding lost hearing by cell transplantation.2007

    • Author(s)
      Sekiya T, Kojima K, Matsumoto M, Holley M, Ito J
    • Journal Title

      Neurosurgery 60(3)

      Pages: 417-433

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve.2007

    • Author(s)
      Sekiya T, Holley MC, Kojima K, Matsumoto M, Helyer R, Ito J.
    • Journal Title

      Eur J Neurosci 25(8)

      Pages: 2307-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Alpha-Klotho asa a regulator of calcium homeostasis.2007

    • Author(s)
      Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, Obuse C, Togashi K, Tominaga M, Kita N, Tomiyama K, Iijima J, Nabeshima Y, Fujioka M, Asato R, Tnanaka S, Kojima K, Ito J, Nozaki K, Hashimoto N, Ito T, Nishio T, Uchiyama T, Fujimori T, Nabeshima Y.
    • Journal Title

      Science (in printing)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] alpha-Klotho asa a regulator of calcium homeostasis.2007

    • Author(s)
      Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, Obuse C, Togashi K, Tominaga M, Kita N, Tomiyama K, Iijima J, Nabeshima Y, Fujioka M, Asato R, Tnanaka S, Kojima K, Ito J, Nozaki K, Hashimoto N, Ito T, Nishio T, Uchiyama T, Fujimori T, Nabeshima Y
    • Journal Title

      Science (accepted)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Recovery of hair cell function after damage induced by gentamicin in organ culture of rat vestibular maculae.2006

    • Author(s)
      Taura A, Kojima K, Ito J, Ohmori H
    • Journal Title

      Brain Res 1098(1)

      Pages: 33-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Innervation of stem cell-derived neurons into auditory epithelia of mice.2005

    • Author(s)
      Matsumoto M., Nakagawa T., Higashi T., et al.
    • Journal Title

      Neuroreport 16 (8)

      Pages: 787-790

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neural connections between embryonic stem cell-derived neurons and vestibular hair cells in vitro.2005

    • Author(s)
      Kim TS., Nakagawa T., Kita T., et al.
    • Journal Title

      Brain research 1057 (1-2)

      Pages: 127-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nuclear translocation of beta-catenin in developing auditory epithelia of mice2005

    • Author(s)
      Takebayashi S., Nakagawa T., Kojima K., et al.
    • Journal Title

      Neuroreport 16 (5)

      Pages: 431-434

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cell transplantation to the auditory nerve and cochlear duct2005

    • Author(s)
      Sekiya T., Kojima K., Matsumoto M., et al.
    • Journal Title

      Experimental neurology 198 (1)

      Pages: 12-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cell transplantation to the auditory nerve and cochlear duct2005

    • Author(s)
      Sekiya T, Kojima K, Matsumoto M, et al.
    • Journal Title

      Experimental neurology 198 (1)

      Pages: 12-24

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Innervation of stem cell-derived neurons into auditory epithelia of mice.2005

    • Author(s)
      Matsumoto M., Nakagawa T., Higashi T., et al.
    • Journal Title

      Neuroreport 16(8)

      Pages: 787-790

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Neural connections between embryonic stem cell-derived neurons and vestibular hair cells in vitro.2005

    • Author(s)
      Kim TS., Nakagawa T., Kita T., et al.
    • Journal Title

      Brain research 1057(1-2)

      Pages: 127-133

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nuclear translocation of beta-catenin in developing auditory epithelia of mice2005

    • Author(s)
      Takebayashi S., Nakagawa T., Kojima K., et al.
    • Journal Title

      Neuroreport 16(5)

      Pages: 431-434

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cell transplantation to the auditory nerve and cochlear duct2005

    • Author(s)
      Sekiya T., Kojima K., Matsumoto M., et al.
    • Journal Title

      Experimental neurology 198(1)

      Pages: 12-24

    • Related Report
      2005 Annual Research Report
  • [Book] 再生医療 : 幹細胞 - 内耳幹細胞 -2006

    • Author(s)
      小島 憲, 伊藤壽一
    • Total Pages
      3
    • Publisher
      メディカルレビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 再生医療 : 幹細胞 -内耳幹細胞-2006

    • Author(s)
      小島憲, 伊藤壽一
    • Total Pages
      3
    • Publisher
      メディカルレビュー社
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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