• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanisms of vestibular compensation and treatment strategy of vertigo

Research Project

Project/Area Number 17591788
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionOsaka University

Principal Investigator

HORII Arata  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 講師 (30294060)

Co-Investigator(Kenkyū-buntansha) KITAHARA Tadashi  Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (30343255)
三代 康雄  大阪大学, 医学系研究科, 講師 (00263260)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsvertigo / vestibular compensation / gene / molecular biology / labyrinthectomy / マイクロアレー / カルシニュリン / microarray / カルシウム
Research Abstract

Inquiries into the neurochemical mechanisms of vestibular compensation, a model of lesion-induced neuronal plasticity, reveal the involvement of both voltage-gated Ca2+ channels (VGCC) and intracellular Ca2+ signaling. Indeed, microarray analysis showed an up-regulation of some calcium signaling-related genes such as the α2 subunit of L-type calcium channels, calcineurin, and plasma membrane Ca2+ ATPase 1 (PMCA1) in the ipsilateral vestibular nuclear complex (VNC) following unilateral vestibular deafferentation (UVD). To further elucidate the role of calcium signaling-related molecules in vestibular compensation, we used a quantitative real-time polymerase chain reaction (PCR) method to confirm the microarray results and investigated changes in expression of these molecules at various stages of compensation (6 h to 2 weeks after UVD). We also investigated the changes in gene expression during Bechterew's phenomenon and the effects of a calcineurin inhibitor on vestibular compensation. … More Real-time PCR showed that genes for the α2 subunit of VGCC, PMCA2,and calcineurin were transiently up-regulated 6 h after UVD in ipsilateral VNC. A subsequent UVD, which induced Bechterew's phenomenon, reproduced a complete mirror image of the changes in gene expressions of PMCA2 and calcineurin seen in the initial UVD, while the α2 subunit of VGCC gene had a trend to increase in VNC ipsilateral to the second lesion. Pre-treatment by FK506,a calcineurin inhibitor, decelerated the vestibular compensation in a dose-dependent manner. Although it is still uncertain whether these changes in gene expression are causally related to the molecular mechanisms of vestibular compensation, this observation suggests that after increasing the Ca2+ influx into the ipsilateral VNC neurons via up-regulated VGCC, calcineurin may be involved in their synaptic plasticity. Conversely, an up-regulation of PMCA2,a brain-specific Ca2+ pump, would increase an efflux of Ca2+ from those neurons and perhaps prevent cell damage following UVD. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (5 results)

All 2007 2006

All Journal Article (5 results)

  • [Journal Article] Unilateral vestibular deafferentation-induced changes in calcium signaling-related molecules in the rat vestibular nuclear complex.2007

    • Author(s)
      C.Masumura, A.Horii, K.Mitani, T.Kitahara, A.Uno, T.Kubo
    • Journal Title

      Brain Research 1138

      Pages: 129-135

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Unilateral vestibular deafferentation-induced changes in calcium signaling-related molecules in the rat vestibular nuclear complex.2007

    • Author(s)
      C.Masumura, A.Horii, K.Mitani, T.Kitahara, A.Uno, T.Kubo
    • Journal Title

      Brain Research. 1138

      Pages: 129-135

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Factors relating to the vertigo control and hearing changes following intratympanic gentamicin for intractable Meniere's disease.2006

    • Author(s)
      A.Horii, T.Saika, A.Uno, S.Nishiike, M.Nishimura, K.Mitani, T.Kitahara, M.Fukushima, A.Nakagawa, C.Masumura, T.Sasaki, K.Kizawa, T.Kuho
    • Journal Title

      Otology and Neurotology 27

      Pages: 896-900

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lesions of the vestibular system disrupt hippocampal theta rhythm in the rat.2006

    • Author(s)
      N.A.Russell, A.Horii, P.F.Smith, C.L.Darlington, D.K.Bilkey
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 4-14

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Fos-enkephalin signaling in the medial vestibular nucleus facilitates vestibular compensation.2006

    • Author(s)
      T.Kitahara, T.Kaneko, A.Horii, M.Fukushima, K.Kizawa-Okumura, N.Takeda, T.Kubo
    • Journal Title

      Journal of Neuroscience Research 83

      Pages: 1573-1583

    • Related Report
      2006 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi