• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

水チャネルの内耳局在と内耳水代謝に対する役割に関する研究

Research Project

Project/Area Number 17591792
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKochi University

Principal Investigator

TAKEDA Taizo  Kochi University, Kochi Medical School, Professor, 医学部, 教授 (50115763)

Co-Investigator(Kenkyū-buntansha) OKADA Teruhiko  Kochi University, Kochi Medical School, Associate Professor, 医学部, 助教授 (00025628)
TAKIGI Akinobu  Kochi University, Kochi Medical School, Assistant Professor, 医学部, 講師 (60243820)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsaquaporin / V2-receptor / water homeostasis / stria vascularis / endolymphatic sac / rat / human / aquaporin 3 / aquaporin 4 / aquaporin 6 / vasopressin / vasopressin type 2 receptor / lithium / collapse
Research Abstract

In order to evaluate the homeostasis of water in the inner ear, localization of AQP-2,-3,-4,-6,-7,-8,-9 and V2-R in the lateral wall of the cochlea were investigated and water homeostasis of the cochlea was discussed.
Molecular biological and histochemistry studies were performed. Animals used were Wistar rats. In molecular biological study, the expression of AQP-2,-3,-4,-6,-7,-8,-9 and V2-R mRNAs was investigated. In histochemistry study, localization of AQP-2,-3,-4,-6,-7,-8,-9 and V2-R was observed by a Confocal laser microscopy.
AQP-2,-3,-4,-6,-7,-9 and V2-R mRNAs were expressed in the rat cochlea. Histochemistry revealed that Proteins of AQP-2,-3, and V2-R were expressed in the basal cells on the stria vascularis. AQP-6 protein was detected in the all cells of the stria vascularis. On the other hand, AQP-4 was not expressed in the lateral wall of the stria vascularis.
AQP-7,-9 were expressed in the apical site of the marginal cells.
AQP-2,-3 and V2-R is localized in the basal cells, located at the boundary between the perilymphatic system and the endolymphatic system. Since osmolality is generally thought to be higher in the endolymphatic compartment, water might enter into the extracellular space via these water channels. Water in the extracellular space is speculated to enter the marginal cells through Na-K-Cl cotransporter and to flow out through AQP-7 and-9 located in the apical membrane.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (7 results)

All 2007 2006 2005

All Journal Article (7 results)

  • [Journal Article] Expression of aquaporin-2, vasopressin type 2 receptor, transient receptor potential channel vanilloid (TRPV)1, and TRPV4 in the human endolymphatic sac.2007

    • Author(s)
      Taguchi D, Takeda T, Kakigi A, Takumida M, Nishioka R, Kitano H
    • Journal Title

      Laryngoscope 117

      Pages: 695-698

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Expression of aquaporin-2,vasopressin type 2 receptor, transient receptor potential channel vanilloid (TRPV)1,and TRPV4 in the human endolymphatic sac.2007

    • Author(s)
      Taguchi D, Takeda T, Kakigi A, Takumida M, Nishioka R, Kitano H.
    • Journal Title

      Laryngoscope. 117

      Pages: 695-698

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparison of dehydration effects of V2-antagonist (OPC-31260) on the inner ear between systemic and round window applications2006

    • Author(s)
      Takeda T, Takeda S, Kakigi A, Okada T, Nishioka R, Taguchi D
    • Journal Title

      Hearing Research 218

      Pages: 89-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A comparison of dehydration effects of V2-antagonist (OPC-31260) on the inner ear between systemic and round window applications2006

    • Author(s)
      Takeda T, Takeda S, Kakigi A, Okada T, Nishioka R, Taguchi D.
    • Journal Title

      Hearing Research 218

      Pages: 89-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of lithium on endolymph homeostasis and experimentally induced endolymphatic hydrops2005

    • Author(s)
      Fukushima K, Takeda T, Kakigi A, Takeda S, Sawada S, Nishioka R, Azuma H, Taguchi D
    • Journal Title

      ORL J Otorhinolaryngology Relat Spec 67

      Pages: 282-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of Lithium on Endolymph Homeostasis and Experimentally Induced Endolymphatic Hydrops.2005

    • Author(s)
      Fukushima K, Takeda T, Kakigi A, Takeda S, Sawada S, Nishioka R, Azuma H, Taguchi D.
    • Journal Title

      ORL 67

      Pages: 282-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of Lithium on Endolymph Homeostasis and Experimentally Induced Endolymphatic Hydrops2005

    • Author(s)
      Fukushima K, Takeda T, Kakigi A, Takeda S, Sawada S, Nishioka R, Azuma H, Taguchi D
    • Journal Title

      ORL J Otorhinolaryngol Relat Spec 67

      Pages: 282-288

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi