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Differentiation of the stem cells from cochlea tissue into cochler sensory epithelial cells.

Research Project

Project/Area Number 17591796
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

OZEKI Masashi  Nagoya City University, Graduate School of Medical Sciences, Research Fellow, 大学院医学研究科, 研究員 (80315887)

Co-Investigator(Kenkyū-buntansha) HIDA Hideki  Nagoya City University, Graduate School of Medical Sciences, Associate, 大学院医学研究科, 助教授 (00305525)
HAMAJIMA Yuki  Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院医学研究科, 助手 (30343403)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsID1(inhibitor of differentiation1) / NF-kappaB / cyclin D1 / cochlear sensory epithelial cells / 内耳多能幹細胞 / セルライン / Notch,HES1,math1,Wnt遺伝子 / 内耳有毛細胞の分化
Research Abstract

Inhibitors of differentiation (Id) play an essential role in the neurogenesis of the central nervous system. However, the expression and function of Id in the development of cochlear sensory epithelial cells have yet to be elucidated. In this study, we demonstrate the Id1 gene was expressed in the rapidly growing otocyst on embryonic day 12 (E12) and in the organ of Corti, spiral ganglions, and stria vascularis on postnatal day 1 (P1) by cellular and molecular biologic techniques. Knockdown of the Id1 gene with short interfering RNA (siRNA) in a cochlear sensory epithelial cell line (OC1) significantly reduced its proliferation, whereas overexpression of Id1 in OC1 significantly increased the proliferation of OC1, suggesting a role of Id1 in the development of cochlear sensory epithelial cells. The proliferative action of Id1 on OC1 was mediated by nuclear factor-kappaB (NF-kappaB) and cyclin D1 (a downstream molecule of NF-kappaB). Blockage of the NF-kappaB activity with pyrrolidine dithiocarbamate (PDTC) or enhancement of the NF-kappaB activity with p65 (a subunit of NF-kappaB) in OC1 significantly inhibited or increased, respectively, the cell proliferation and transcription of cyclin D1 induced by Id1. Truncation of the NF-kappaB binding site in the cyclin D1 promoter fully abrogated the transcription of cyclin D1, suggesting that the cyclin D1 transcription is dependent on NF-kappaB. We concluded from this study that Id1 induces the proliferation of OC1 via the NF-kappaB/cyclin D1 pathway.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006 2005

All Journal Article (11 results)

  • [Journal Article] Idl induces the proliferation of cochlear sensory epithelial cells via the nuclear factor- κB/cyclin D1 pathway in vitro2007

    • Author(s)
      Ozeki M
    • Journal Title

      Journal of neuroscience research 85

      Pages: 515-524

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Id1 induces the proliferation of cochlear sensory epithelial cells via the nuclear factor-K B/cyclin D1 pathway in vitro.2007

    • Author(s)
      Ozeki M
    • Journal Title

      Journal of Neuroscience Research 85

      Pages: 515-524

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pneumococcal peptidoglycan-polysaccharides induce the expression of interleukin-8 in airway epithelial cells by way of nuclear factor-kappaB, nuclear factor interleukin-6m or activation protein-1 denendent mechanisms.2007

    • Author(s)
      Tsuchiya K
    • Journal Title

      Laryngoscope 117・1

      Pages: 86-91

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 中耳炎におけるLevofloxacinの投与法についての検討2006

    • Author(s)
      小関 晶嗣
    • Journal Title

      日本耳鼻咽喉科感染症研究会会誌 24

      Pages: 91-93

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Investigation of an effective administration of levofloxacin for otitis media.2006

    • Author(s)
      Ozeki M
    • Journal Title

      24

      Pages: 91-93

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Id1 induces the proliferation of Cochlear sensory epithelial cells via the nuclear factor-kB/Cyclin D1 pathway in Vitro2006

    • Author(s)
      Ozeki M
    • Journal Title

      Jounal of Neuroscience Reserch 85

      Pages: 515-522

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization of inhibitor of differentiation(Id3)gene expression in the developing cochlear tissue of rats.2005

    • Author(s)
      Ozeki H
    • Journal Title

      Acta Oto-Laryngologica 125

      Pages: 244-249

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of the integrin genes in the developing cochlea of rats2005

    • Author(s)
      Toyama K
    • Journal Title

      Hearing Research 201

      Pages: 21-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of inhibitor of differentiation (Id3) gene expression in the developing cochlear tissue of rats.2005

    • Author(s)
      Ozeki M
    • Journal Title

      Acta Oto-Laryngologica 125

      Pages: 244-249

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Expression of the integrin in the developing cochlea of rats.2005

    • Author(s)
      Toyama K
    • Journal Title

      Hearing Research 201

      Pages: 21-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of the integrin genes in the developing cochlea of rats.2005

    • Author(s)
      Toyama K
    • Journal Title

      Hearing Res 201

      Pages: 21-26

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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