| Project/Area Number |
17591800
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kitasato University |
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Principal Investigator |
NAKAYAMA Meijin Kitasato University, School of Medicine, Otorhinolaryngology, Assistant Professor (20207955)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Yuichi Kitasato University, School of Allied Health Science, Molecular Diagnostics, Associate Professor (30178793)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Cadherin / Laminin / Carcinoma in situ / Neuroleukin / DJ-1 mRNA / Hypopharyngeal cancer / Dysplasia / Autocrine Motility Factor / DJ-1 |
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| Research Abstract |
To elucidate the topical features of cancer invasion within the whole-organ sections of hypopharyngeal cancers, stainability of 1) E-cadherin and β-catenin, 2) Neuroleukin and DJ-1 mRNA expression, and 3) Laminin-5 y2 chain expression were analyzed.
1) Reduced expression of E-cadherin and β-catenin was observed at the tumor edge of cartilage invasion and at the invasive front. This fact suggested an increased activity and invasiveness occurred in the cancer tissues especially at these sites. Stainability did not significantly correlate with prognosis.
2) Increased stainability was observed in Neuroleukin and DJ-1 mRNA expressions. Further improvement of staining method should be contemplated.
3) Laminin-5 is the most promising factor at this point. Increased stainability was observed at invasive front and did correlate well with prognosis.
Cell adhesion molecules became much useful when combined with the prognostic factors elicited from H-E stained whole-organ serial sections in hypopharyngeal cancers. Further investigations, including other morphogenetic regulators, will be needed to fully elucidate the tumor behavior of hypopharyngeal cancer.
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