Analysis of inner ear specific autoantibody in experimental autoimmune labyrinthitis model
| Project/Area Number |
17591803
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
TOMIYAMA Shunichi Nippon Medical School, Otorhinolaryngology, Professor (00094665)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Kenichi Nippon Medical School, Otorhinolaryngology, Lecturer (80281434)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | inner ear autoimmune disease / inner ear specific autoantibody / animal model / SDS-PAGE / whole gel eluter / western blotting / proteome profiling / inner ear specific auto-antigen / 自己免疫病 / 電気泳動 / ウェスタンブロット |
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| Research Abstract |
In order to establish experimental inner ear specific autoimmune labyrinthitis in mice, we designed to recover inner ear specific proteins from crude inner ear proteins by SDS-technique and proteome profiling. Thirty partitioned inner ear proteins according to molecular size were harvested through elution of crude inner ear antigens on Whole Gel Eluter. Serum from mice sensitized with 19 out of 30 partitions produced antibodies against bovine inner ear proteins by western blotting and 7 positive different bands were recognized. Proteome profiling of several partitions that demonstrated clear positive bands were selectively carried out. Partition number 3, 10 and 11 hit only COCH protein. Partition number 18, 22 and 24 hit non specific inner ear proteins. This study succeeded in isolation of inner ear specific proteins, suggesting that development of inner ear specific autoimmune animal model is possible to elucidate pathogenesis of autoimmune inner ear disease.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Expression of cochline in the vestibular organ of rats2005
Author(s)
Tetsuo, Ikezono, Susumu, Shindo, Masamichi, Ishizaki, Lishu, Li, Shuncihi, Tomiyama, Masaya, Takumida, Ruby, Pawankar, Atsushi, Watanabe, Akihiko Saito, Thshiaki, Yagi
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] 内耳自己免疫病の治療経験2007
Author(s)
富山 俊一
Organizer
第17回日本耳科学会
Place of Presentation
福岡
Year and Date
2007-10-19
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Inner ear autoimmune injured pathogensis in acute low tone sensory nerve heraring loss2007
Author(s)
Shunichi, Tomiyama, Akihiko, Saito, Chika, Ozu, Manabu, Nonaka, Atuko, Sakanushi, kenichi, Shimada
Organizer
108th the Otorhinolaryngology society of Japan
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Clinical experience of Autoimmune inner ear disease2007
Author(s)
Shunichi, Tomiyama, Akihiko, Saito, Nozomu, Ogiwara, Chika, Ozu, Manabu, Nonaka, Mayumi, Kunitomo
Organizer
17th Otological society of Japan
Description
「研究成果報告書概要(欧文)」より
Related Report
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