| Project/Area Number |
17591823
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
FUTAGAMI Soh Tokyo Medical and Dental University Graduate School, Ophthalmology and Visual Science, Lecturer, 医学部附属病院, 非常勤講師 (70301158)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHNO Kyoko (MATSUI Kyoko) Tokyo Medical and Dental University Graduate School, Ophthalmology and Visual Science, Assistant Professor, 大学院医歯学総合研究科, 准教授 (30262174)
MOCHIZUKI Manabu Tokyo Medical and Dental University Graduate School, Ophthalmology and Visual Science, Professor & Chairman, 大学院医歯学総合研究科, 教授 (10010464)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | pathologic myopia / mechanical stress / choroidal neovascularization / retinal pigmented epithelium / VEGF / PEDF / 網膜脈絡膜 / 血管新生 / 網膜色素上皮 / 機械的伸展 / 血管内皮増殖因子 / 色素上皮由来因子 |
|---|
| Research Abstract |
Pathologic myopia is a major cause of the blindness in many developed countries. Among various fundus lesions in highly myopic eyes, choroidal neovascularization (CNV) is the most common cause for visual impairment.
We hypothesized that mechanical stretch loaded onto retinal pigmented epithelial (RPE) cells due to axial elongation of the eyeball causes the change of expression of angiogenesis-related factors, and thus causes CNV.
To prove this, we cultured human RPE cells in differentiated condition on laminin-coated dish. Using FlexerCell machine, we loaded 15% stretch on cultured RPE cells for 24 hours at intervals of 30 minutes. The results demonstrated that vascular endothelial growth factor (VEGF) and monocytes chemoattractant factor-1 (MCP-1) were upregulated both at mRNA and protein levels after mechanical stretch. In contrast, pigment epithelium-derived factor (PEDF) was decreased at protein level. This suggests that mechanical stress causes unbalance of angiogenesis-related factors released from RPE cells, which might be important for the development of CNV in pathologic myopia.
I also investigated the cause of CNV development in highly myopic eyes from the point of ocular circulation using ICG angiography. The results showed that choroidal circulation dramatically changes with time in highly myopic eyes, and the remodeling of choroidal vasculature might be related to the CNV development.
|
