| Project/Area Number |
17591832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
KUSAKA Shunji Osaka University, Graduate School of Medicine, Associate Professor (60260387)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Yusuke Osaka University, Graduate School of Medicine, Assistant Professor (20362717)
SAISHIN Yoshitsugu Osaka University, Graduate School of Medicine, Assistant Professor (30379193)
FUJIKADO Takashi Osaka University, Graduate School of Medicine, Professor (50243233)
TANO Yasuo Osaka University, Graduate School of Medicine, Professor (80093433)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | retinopathy of prematurity / VEGF / eDNA microarray / neovasucuralization / model mouse |
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| Research Abstract |
Background/aims : To investigate the correlation between the clinical course and gene expression pattern in murine oxygen-induced retinopathy (OIR), a common model of retinopathy of prematurity (ROP).
Methods: OIR was induced in C57BL/6N mice by placing postnatal day 7 (P7) pups in 75% oxygen for 5 days. The clinical course of OIR was evaluated on retinal flat-mounts with FITC-conjugated dextran perfusion from P12 to P21. The expression values of 94 genes, selected by microarray analysis, were determined daily from P12 through P21 by RT-PCR with TagMan (R) low-density array (TLDA) and analyzed by hierarchical clustering.
Results: TLDA cluster analysis showed the homology of gene expression pattern between P12 and P13, and between P16 and P17. Many genes associated with inflammation were up-regulated from P12 and P13, when the degrees of both central avascular area and central vasoconstriction were maximal, and the up-regulation of the genes continued to P21. Several representative genes associated with angiogenesis, e.g., vascular endothelial growth factor-A and angiopoietin-2, were most up-regulated at P16 and P17, when extraretinal neovascularization became most noticeable.
Conclusion: The gene expression pattern was well-correlated with the clinical presentation in murine OIR. These findings will contribute to understand the pathological condition involved in ROP.
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