| Project/Area Number |
17591846
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Chiba University (2006)
Sapporo Medical University (2005) |
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Principal Investigator |
MITAMURA Yoshinori Chiba University, Graduate School of Medicine, Associate Professor, 大学院医学研究院, 助教授 (30287536)
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| Co-Investigator(Kenkyū-buntansha) |
OHGURO Hiroshi Sapporo Medical University, Professor, 医学部, 教授 (30203748)
田下 亜佐子 札幌医科大学, 医学部, 助手 (50347176)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Diabetic retinopathy / Neovascularization / Transcriptional factor |
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| Research Abstract |
To assess the potential role of transcriptional factor in nevascularization of proliferative diabetic retinopathy (PDR), we examined the expression of activator protein-1 (AP-1) and nuclear factor kappa B (NFkB) in epiretinal membrane (ERM) of PDR and performed in vitro studies.
Expression of AP-1 and NFkB in PDR ERMs
1) AP-1 mRNA was detected in all (100%) of the 19 eyes with PDR, but in only 7 (44%) of the 16 eyes with idiopathic ERM (P<0.05). NFkB mRNA was detected in 14 (74%) of the 19 eyes with PDR, but in only 6 (38%) of the 16 eyes with idiopathic ERM (P<0.05).
2) AP-1 protein was detected in PDR ERMs and was coexpressed with the glial-specific marker vimentin. Also, NFkB protein was detected in PDR ERMs and was coexpressed with vimentin and MCP-1.
in vitro study
1) Glycated albumin stimulation significantly increased AP-1 phosphorylation in glial cells. Also, glycated albumin stimulation induced NFkB activationation and translocation to nuclei in glial cells.
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