| Project/Area Number |
17591848
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
HARA Hideaki Gifu Pharmaceutical University, Professor, 薬学部, 教授 (20381717)
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| Co-Investigator(Kenkyū-buntansha) |
ARAIE Makoto Tokyo University, School of Medicine, Professor, 医学部付属病院眼科, 教授 (00092122)
IMAMURA Kazuyuki Riken, Brain Science Institute, Sub Reader, 視覚神経回路モデル研究チーム, 副チームリーダー (30203326)
WATANABE Yasuyoshi Osaka City Univ., Graduate School of Medicine, Professor, 大学院医学研究科・システム神経科学, 教授 (40144399)
SHIMAZAWA Masamitsu Gifu Pharmaceutical University, Associated Professor, 薬学部, 講師 (80381721)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Glaucoma / High intraocular pressure / Optic nerve damage / Ophthalmology / Fundal change / Early therapy / Retinal damage / PET |
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| Research Abstract |
1) The purpose was to investigate time-dependent, exofocal alterations in the lateral geniculate nucleus (LGN) following NMDA-induced retinal damage in mice. Retinal damage was noted at 1 day after NMDA injection. At 7 days after NMDA injection, atrophy of the optic tract was observed on the contralateral side. In the contralateral dorsal LGN (d-LGN) and contralateral ventral LGN-lateral (vLGN-1), neurons were first decreased in number at 90 and 180 days, respectively, after NMDA injection, and neuronal cell-size was first decreased at 30 and 7 days, respectively, after NMDA injection. During this neuronal degeneration, expressions of glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) were increased in the contralateral dLGN and vLGN-1, with some GFAP-positive astroglial cells also exhibiting BDNF. After NMDA injection, time-dependent, exofocal alterations occurred along the retinogeniculate pathway (from retina to LGN), and the degree of damage in LGN was region-dependent. Increased expressions of GFAP and BDNF in the affected LGN regions may play some roles in the retrograde cell-death mechanism in this nucleus.
2) Alongside the IOP elevation, time-related changes in optic disc topography and RNFL thickness were demonstrated in monkey eyes using HRT and GDx. HRT (rim and cup) parameters showed good correlations with histological RNFL thickness, and significant interrelations.
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