| Project/Area Number |
17591858
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
MIMURA Osamu Hyogo College of Medicine, Faculty of Medicine, Professor (60157584)
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| Co-Investigator(Kenkyū-buntansha) |
DEZAWA Mari Tohoku University School of Medicine, Faculty of Medicine, Professor (50272323)
ISHIKAWA Hiroto Hyogo College of Medicine, Faculty of Medicine, Research Associate (10434945)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Amblyopia / Cell transplantation / Bone marrow stromal cells / Dopamine / Nerve regeneration / Regenerative Medicine |
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| Research Abstract |
We showed the analysis of rat deprivation amblyopia in this research. In 2005, we made the amblyopia models, differentiation of bone marrow stromal cells (BMSc) to dopaminergic neuronal cells (DNc), and cell transplantation via cerebrospinal fluid to rat amblyopia models. In 2006, we analyzed these models using antibody array and PCR. Antibody array showed up-regulation of Tyrosine hydroxylase (Th) and down-regulation of signal proteins for apoptotic and MAP kinase signaling system in amblyopic brain. PCR study showed down-regulation of Th in amblyopic retina, and down-regulation signal proteins for apoptotic and MAP kinase signaling system in both amblyopic brain and retina. In the cell transplantation group, PCR study showed up-regulation of these proteins, neurofilament and GFAP. These results suggest that the BMSc derived DNc transplantation into rat amblyopia model is effective in up-regulation of Th, anti-apoptotic pathway and the survival of neuronal cells. In the future, there is a necessity for examining the effect of cell transplantation compared with the administration of Levodopa.
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