| Project/Area Number |
17591878
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
MIZUNO Hiroshi Nippon Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80343606)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Rei Nippon Medical School, Faculty of Medicine, Assistant Professor, 医学部, 講師 (70398866)
KOIKE Sachiko Nippon Medical School, Faculty of Medicine, Research Associate, 医学部, 助手 (90386227)
HYAKUSOKU Hiko Nippon Medical School, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (00165135)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | mesenchymal stem cells / adipose tissue / periodontal tissue / periodontal diseases / regenerative medicine / tissue engineering |
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| Research Abstract |
A number of surgical techniques have been developed to promote periodontal tissue regeneration. However, they are ineffective or impractical. Bone marrow-derived stem cells have been shown to promote periodontal tissue regeneration. In this study, we sought to determine whether adipose-derived stem cells (ASCs) can also promote periodontal tissue regeneration. ASCs were isolated from a Wistar rat, passaged twice, mixed with platelet-rich plasma (PRP) obtained from inbred rats, and implanted into the periodontal tissue defect that was generated in the test rats. Tissue specimens were harvested after two, four, and eight weeks for histological analysis. Rats that received PRP only or were not implanted served as control groups. A small amount of alveolar bone regeneration was observed two and four weeks after ASC/PRP implantation. Moreover, eight weeks after implantation, a periodontal ligament (PDL)-like structure was observed along with alveolar bone. In addition, GFP-labelled ASCs existed in regenerated alveolar bone and PDL-like structure were also stained positively for anti osteocalcin antibodies, suggesting that these ASCs were directly differentiated into osteocytes and PDL constructs.
We extended these experimental approaches to canine model to confirm that periodontal tissue regeneration could be made in a same manner. 4 weeks after implantation, dental X ray showed that lucency of the bony defect was decreased compared to the control group. Moreover, immunohistochemical analysis demonstrated that cells stained positively for anti-osteocalcin and anti-type I collagen antibodies were found.
These observations suggest that ASCs can promote periodontal tissue regeneration in vivo.
Since large amounts of human lipoaspirates are readily available and their procurement induces only low morbidity, ASCs may be useful in future clinical cell-based therapy for periodontal disease.
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