| Project/Area Number |
17591895
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | SHOWA University |
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Principal Investigator |
SATOH Kazue Showa-University, Medicine, Visiting Professor, 医学部, 客員教授 (90053941)
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| Co-Investigator(Kenkyū-buntansha) |
ARUGA Tohru Showa-University, Medicine, Professor, 医学部, 教授 (40266086)
SIODA Seiji Showa-University, Medicine, Professor, 医学部, 教授 (80102375)
DOHI Kenji Showa-University, Medicine, Assistant Professor, 医学部, 講師 (20301509)
大滝 博和 昭和大学, 医学部, 助手 (20349062)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | electron spin resonance (ESR) / free radical / alkoxyl radical / brain injury / reactive oxygen species / spin trapping / lipid hydroperoxide / oxidative stress / アルコキシルラジカル |
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| Research Abstract |
Oxygen Free radicals have important roles of various acute neuronal conditions. Especially neuroemergency diseases such as neurotrauma, stroke, traumatic brain injury and ischemia after cardiac arrest are strongly related with oxidative stress. However, the study of free radicals in body fluid is currently severely hampered by technical difficulties in their detection. We have developed an ex vivo electron spin resonance (ESR) method, that employs 5,5-dimethyl-l-pyrroline-N-oxide (DMPO) as a spin trap, to measure the alkoxyl radical (^●OR) in human blood. We found that this method can detect ^●OR produced by lipid peroxidation, which is caused by reactive oxygen species. Blood samples were collected from the catheter of the internal jugular bulb. The control samples were also collected from healthy volunteers. Alkoxyl radical level of all patients with neuroemergency deseases, who were admitted to Showa University Hospital Emergency Centre, were elevated in comparison with control. Alkoxyl radical level was shown the condition of patients and was suppressed after administration of Edaravone, a novel synthetic free radical scavenger shown to exert neuroprotective effect in acute ischemic stroke. This method is useful to monitor the free radical level immediately at bedside and it will enable to detect the oxidative stress and the severity of brain damage. In the future we will examine to prevention and the deletion using antioxidant or antioxidative food. This study was supported by ethical committee of Showa University.
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