Free Radical Monitoring of Brain Injury and their Basic Studies
| Project/Area Number |
17591895
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | SHOWA University |
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Principal Investigator |
SATOH Kazue Showa-University, Medicine, Visiting Professor, 医学部, 客員教授 (90053941)
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| Co-Investigator(Kenkyū-buntansha) |
ARUGA Tohru Showa-University, Medicine, Professor, 医学部, 教授 (40266086)
SIODA Seiji Showa-University, Medicine, Professor, 医学部, 教授 (80102375)
DOHI Kenji Showa-University, Medicine, Assistant Professor, 医学部, 講師 (20301509)
大滝 博和 昭和大学, 医学部, 助手 (20349062)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | electron spin resonance (ESR) / free radical / alkoxyl radical / brain injury / reactive oxygen species / spin trapping / lipid hydroperoxide / oxidative stress / アルコキシルラジカル |
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| Research Abstract |
Oxygen Free radicals have important roles of various acute neuronal conditions. Especially neuroemergency diseases such as neurotrauma, stroke, traumatic brain injury and ischemia after cardiac arrest are strongly related with oxidative stress. However, the study of free radicals in body fluid is currently severely hampered by technical difficulties in their detection. We have developed an ex vivo electron spin resonance (ESR) method, that employs 5,5-dimethyl-l-pyrroline-N-oxide (DMPO) as a spin trap, to measure the alkoxyl radical (^●OR) in human blood. We found that this method can detect ^●OR produced by lipid peroxidation, which is caused by reactive oxygen species. Blood samples were collected from the catheter of the internal jugular bulb. The control samples were also collected from healthy volunteers. Alkoxyl radical level of all patients with neuroemergency deseases, who were admitted to Showa University Hospital Emergency Centre, were elevated in comparison with control. Alkoxyl radical level was shown the condition of patients and was suppressed after administration of Edaravone, a novel synthetic free radical scavenger shown to exert neuroprotective effect in acute ischemic stroke. This method is useful to monitor the free radical level immediately at bedside and it will enable to detect the oxidative stress and the severity of brain damage. In the future we will examine to prevention and the deletion using antioxidant or antioxidative food. This study was supported by ethical committee of Showa University.
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Report
(3 results)
Research Products
(69 results)
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[Journal Article] Influence of Oxidative Stress on Fusion of Pre-Synaptic Plasma Membranes of the Rat Brain with Phosphatidyl Choline Liposomes, and Protective Effect of Vitamin E :2006
Author(s)
Omoi N, Arai M, Saito M, Takatsu H, Sibata A, Fukuzawa K, Satoh K, Abe K, Fukui K, Urano S
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Journal Title
J Nutri Sci Vitamin 52
Pages: 248-255
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Influence of SNPs in cytokine-related genes on the severity of Food allergy and atopic eczema in children ;2006
Author(s)
Negoro T, Orihara K, Irahara T, Nishiyama H, Hagiwara K, Nishida R, Takagi H, Satoh K, Yamamoto Y, Shimizu S, Hagiwara T, Ishi M, Tanioka T, Nakano Y, Takeda K, Yoshimura I, Iikura Y, Tobe T.
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Journal Title
Pediatr Allergy Immunol 17
Pages: 583-590
Description
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[Journal Article] Induction of Tumor-specific Cytotoxicity and Apoptosis by Doxorubicin.2005
Author(s)
Suzuki F., Hashimoto K., Kikuchi H., Nishikawa H., Matsumoto H., Shimada J., Kawase M., Sunaga K., Tsuda T., Satoh K., Sakagami H.
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Journal Title
Anticancer Res. 25
Pages: 887-894
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tumor-specific Cytotoxicity of 3,5-Dibenzoyl-1,4-dihydropyridines.2005
Author(s)
Morshed S.M.D., Hashimoto K., Murotani Y., Kawase M., Shan A., Satoh K., Kikuchi H., Nishikawa H., Maki J., Sakagami H.
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Journal Title
Anticancer Res. 25
Pages: 2033-2038
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibition of LPS-stimulated NO Production in Mouse Macrophage-like Cells by Azulenequinones.2005
Author(s)
Nishishiro M., Arikawa S., Wakabayashi H., Hashimoto K., Satoh K., Yokoyama K., Unten S., Kakuta H., KuriharaT., Motohashi N., Sakagami H.
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Journal Title
Anticancer Res. 25
Pages: 4157-4164
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cytotoxicity and radical modulating activity of Moxa smoke.2005
Author(s)
Sakagami H, Matsumoto H, Satoh K, Shioda S, Chowdhury SA, Hashimoto K, Kikuchi H, Nishikawa H, Terakubo S, Shoji Y, Nakashima H, Shimada J
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Journal Title
In Vivo 19
Pages: 391-398
Description
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