| Project/Area Number |
17591897
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Nihon University |
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Principal Investigator |
KINOSHITA Kosaku Nihon University, School of Medicine, Associate Professor, 医学部, 准教授 (90260968)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Atsushi Nihon University, School of Medicine, Assistant, 医学部, 助手 (40339320)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Hyperglycemia / Traumatic brain injury / Endothelial injury / Cytokine / Tumor necrotic factor-alpha / Tumor necrotic factor-alpha / Lipopolysaccharide |
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| Research Abstract |
Background.
This study evaluated the changes in chemokine interleukin (IL)-8 production from endothelial cells under various hyperglycemic conditions and investigated whether the hyperglycemia associated with the acute inflammatory response could enhance the IL-8 production from the endothelial cells.
Materials and Methods.
Human umbilical endothelial cells (HUVECs) were seeded at a concentration of 1×105 cells/well and cultured. The culture medium was replaced with Medium 199 containing various concentrations of glucose (final glucose concentration of culture medium was 100, 200, 300, 400, 500 mg/dL ; n= 7 each) with or without 100 ng of TNF-alpha. After 12 or 24 h at 37℃, the supernatants were collected from the cultures and stored at-80℃ until cytokine assay. IL-8 levels of the samples from the supernatants were quantified using a commercially available ELISA kit.
Results.
The IL-8 production by the HUVECs was significantly higher in the high glucose culture than in the control culture (glucose concentration of 100 mg/dL) (P<0.05). Moreover, the hyperglycemia associated with elevated TNF-alpha was found to enhance the level of IL-8 production by the HUVECs cultured at all glucose concentrations and over both time courses, compared to the control (P<0.05).
Conclusions.
In this study we observed a significant augmentation of IL-8 production by endothelial cells during short-term hyperglycemia, and a similar but significant stronger augmentation was obtained through TNF treatment. These findings suggest that the hyperglycemia associated with acute inflammatory response after trauma may run into the patients at high risk for secondary tissue damage.
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