| Project/Area Number |
17591898
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Tokyo medical and dental university (2006-2007)
Nippon Medical School (2005) |
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Principal Investigator |
AIBOSHI Junichi Tokyo medical and dental university, university hospital, junior associated professor (50256913)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Yasuhiro Nippon medical school, medical science, professor (70125079)
KOIKE Kaoru Kyoto university, medical science, professor (10267164)
HIRAKAWA Keiko Nippon medical school, school of medicine, assistant professor (30165162)
KOBAYASHI Tetsuyuki Ochanomizu university, faculty of science, professor (50178323)
MASUNO Tomohiko Nippon medical school, school of medicine, assistant professor (00318528)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥2,650,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Lyso-phospholipids / Mesenteric lymph / Hemorrhagic shock / Blood substitute / Stored blood / Multiple organ dysfunction / Neutrophils / Nuclear magnetic resonance spectroscopy / 腸間膜リンパ液 / 脂質メディエータ / 肺傷害 / lyso-phosphatidylethanolamine / lyso-phosphatidylcholine |
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| Research Abstract |
1. Identification of inflammatory mediators in mesenteric lymph following hemorrhagic shock
We found six inflammatory mediators, tumor necrosis factor, cytokine-induced neutrophil chemoattractant-1, linoleic lyso-phosphatidylethanolamine (LPE), linoleic lyso-phosphatidylcholine (LPC), arachidonic LPE, and arachidonic LPC, in mesenteric lymph following hemorrhagic shock.
2. Analysis of biological activities of lipid mediators identified in mesenteric lymph
We evaluated biological activities for human neutrophils (superoxide production, elastase release, and transmigration) of lyso-phospholipids which we discovered in mesenteric lymph. Four lyso-phospholipids induced biological activities to human neutrophils in dose-dependent manner. This was the first report of the presence of inflammatory mediators in mesenteric lymph. It is expected that elucidation of the site and mechanism of production links to the strategies for multiple organ failure (MOF) after hemorrhagic shock.
3. Biological acti
… More
vity of mesenteric lymph after transfusion of stored blood for hemorrhagic shock
We measured the biological activity (superoxide production of human neutrophil) of mesenteric lymph from rats transfused with stored blood. Compared to rats transfused with fresh blood, mesenteric lymph in stored blood group significantly increased superoxide production. On the other hand, Mesenteric lymph from rats transfused with blood substitute induced the significant decrease in superoxide production. Consequently, it is possible that blood substitute attenuates lung injury and systemic inflammatory response syndrome following hemorrhagic shock.
4. The effect of blood substitute on the intestinal oxygen metabolism after hemorrhagic shock
To evaluate the effect of blood substitute on the intestinal oxygen metabolism, we measured lactate and alanine, as a maker of tissue oxygen metabolism, in the intestine exposed to hemorrhagic shock with nuclear magnetic resonance spectroscopy. Blood substitute improved the intestinal oxygen metabolism equal to fresh blood. Previous studies reported that stored blood was associated with the intestinal ischemia and MOF. Therefore, blood substitute may be one of strategy for MOF. Less
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