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Expression of connective tissue growth factor CTGF in the callus during bone regeneration and application for acceleration of regeneration.

Research Project

Project/Area Number 17591908
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOkayama University

Principal Investigator

YAMAAI Yuichiro  Okayama University Graduate School, research assistant, 大学院医歯薬学総合研究科, 助手 (00158057)

Co-Investigator(Kenkyū-buntansha) NAKANISHI Tohru  Shujitsu University, Professor, 薬学部, 教授 (30243463)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCCN / CTGF / osteoclast / callus / bone regeneration / apoptosis / hypertrophic chondrocyte / chemotaxis / mouse / HGF / c-Met / 肥大軟骨細胞 / CTGFノックアウトマウス / 遺伝子発現 / 骨再生
Research Abstract

The utility of the endochondral ossification factor CTGF has pointed out in many cases. We investigated to analyze the role of CTGF for the application to accelerate bone regeneration in vivo. The expression of CTGF was found as a band in the hypertrophic chondrocytes during differentiation of proliferative chondrocytes to the hypertrophic chondrocytes. No difference has been found on the angiogenesis in the bone marrow, on the number of monocytes or megakaryocytes in developing liver of CTGF gene targeted mouse embryos to compared with the wild type embryos. However, the morphology of the long bone of CTGF targeted mice was different from that of the wild type. The serious cleft palate, enlarged hypertrophic zone, developmental delay of ossification and the kinks of long bone have observed in the mutants. These morphological abnormalities might be caused by abnormal arrangement of the osteoclasts. The osteoclasts concentrate to the apoptotic zone of the hypertrophic chondrocytes generally, however, the osteoclasts did not concentrate to the apoptotic zone. The femoral bone was fractured to prepare the callus to analyze the behavior of the osteoclasts. The osteoclasts were scattered around the callus for first 1 week. However, the osteoclasts were rearranged rectangular to the axis and then the absorption of callus cartilage by the osteoclasts was occurred actively. This result indicates that the behavior of osteoclasts affects on the ossification. To confirm the relationship between hypertrophic chondrocytes and the osteoclasts, the combination culture of hypertrophic chondrocytes with the osteoclasts was performed to add anti CTGF and anti c-Met antibodies in the medium. After 24 hours, cells were fixed and stained by TRAP method. Interestingly, the osteoclasts behavior and proliferation were activated with addition of anti CTGF. This result is opposite to the observation in vivo.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (6 results)

All 2005

All Journal Article (6 results)

  • [Journal Article] Gene expression of connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant mice in late stage of embryonic development.2005

    • Author(s)
      Yamaai T, Nakanishi T, Asano M, Nawachi K, Yoshimichi G, Ohyama K, Komori T, Sugimoto, T Takigawa M
    • Journal Title

      J Bone Miner Metab. 23(4)

      Pages: 280-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells.2005

    • Author(s)
      Asano M, Kubota S, Nakanishi T, Nishida T, Yamaai T, Yosimichi G, Ohyama K, Sugimoto T, Murayama Y, Takigawa M
    • Journal Title

      Cell Commun Signal. Oct 5

      Pages: 1-11

    • NAID

      120002311460

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene expression of connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfal-null mutant mice in late stage of embryonic development.2005

    • Author(s)
      Yamaai T, Nakanishi T, Asano M, Nawachi K, Yoshimichi G, Ohyama K, Komori T, Sugimoto T, Takigawa M.
    • Journal Title

      J Bone Miner Metab. 23(4)

      Pages: 280-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells.2005

    • Author(s)
      Asano M, Kubota S, Nakanishi T, Nishida T, Yamaai T, Yosimichi G, Ohyama K, Sugimoto T, Murayama Y, Takigawa M.
    • Journal Title

      Cell Commun Signal.

    • NAID

      120002311460

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene expression connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant mice in late stage of embryonic development2005

    • Author(s)
      Yamaai T, Nakanishi T, Asano M, Nawachi K, Yoshimichi G, Ohyama K, Komori T, Sugimoto T, Takigawa M.
    • Journal Title

      J Bone Miner.Metab. 23(4)

      Pages: 280-288

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells.2005

    • Author(s)
      Asano M, Kubota S, Nakanishi T, Nishida T, Yamaai T, Yosimichi G, Ohyama K, Sugimoto T, Murayama Y, Takigawa M.
    • Journal Title

      Cell Commun.Signal Oct. 5

      Pages: 3-11

    • NAID

      120002311460

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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