| Project/Area Number |
17591919
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
TOYOSHIMA Kuniaki Kyushu Dental College, School of Dentisry, Professor (10112559)
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| Co-Investigator(Kenkyū-buntansha) |
SETA Yuji Kyushu Dental College, School of Dentisry, Associate Professor (90291616)
TOYONO Takashi Kyushu Dental College, School of Dentisry, Assistant Professor (10311929)
KATAOKA Sinji Kyushu Dental College, School of Dentisry, Assistant Professor (80364149)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | taste bud / taste transduction / zinc / transcription / promoter / umami / sweet / taste sensation / 免疫組織化学 / II型細胞 / P450scc / P450aroma / プレグネノロン / エストロゲン / RT-PCR / III型細胞 / シナプトタグミン / セロトニン |
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| Research Abstract |
Taste disorders have been linked to zinc deficiency. Zinc plays structural roles in so called Zinc fingers. Zinc fingers are exploited by transcription factors for interacting with DNA and regulating the activity of genes. Although the mechanisms of transcriptional regulation of the taste receptor genes have not been revealed, it is possible that the zinc is concerned with the transcriptional regulation of taste receptor genes. Therefore, we examined the molecular mechanisms regulating the promoter of human umami and sweet receptor, T1R3 gene in a human intrahepatic bile duct carcinoma cell line, H_uCCT1. The luciferase reporter assay showed that the 226-bp region upstream of the ATG had promoter activity.
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