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Elastic System Fibers Formation and Degradation in the Periodontal Tissues

Research Project

Project/Area Number 17591920
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

YAJIMA Toshihiko  Health Sciences University of Hokkaido, School of Dentistry, Professor (10018749)

Co-Investigator(Kenkyū-buntansha) IRIE Kazuharu  Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (70223352)
TSURUGA Eichi  Fukuoka Dental University, School of Dintistry, Associate Professor (30295901)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordselastic system fibers / microfibrils / elastin / fibrillin / MAGP-1 / MMP-2 / periodontal tissues / fibroblasts / フィブリリン-2 / MMP inhibitor / TIMP-2 / フィビユリン-5 / フィブリン
Research Abstract

Elastic system fibers, comprised of microfibrils and tropoelastin, are extracellular components of periodontal tissue. Elastogenesis requires a microfibril template onto which tropoelastin is deposited. The microfibrils, which are made up mainly of fibrillins, microfibril-associated glycoporoteins (MAGPs) and fibulins, are thought to serve as a template structure that guides tropoelastin desposition.
In this study, we used the RNA interference (RNAi) technique to estanblish MAGP-1 and fibrillin-2 gene-specific knock-downs individually in human gingival fibroblasts (HGF). We then examined the extracellular deposition of tropoelastin by western blotting. These two genes were specifically suppressed to <30% of the control level, and this was responsible for the diminution of tropoelastin deposition. An immunof;uorescence study also cinfrimed that RNAi-mediated down-regulation of MAGP-1 or fibrillin-2 led to the loss of tropoelastin immunoreactivity. These results suggest that MAGP-1 and fibrillin-2 are associated with the extracellular deposition of tropoelastin during elastic fiber formation in HGF in vitro.
The process of elastic system fibersr remodeling is not fully understood. We examined whether matrix metaroproteinases (MMPs) are involved in remodeling of fibrillins by HGF and human periodontal ligament fibroblasts (HPLF). Active MMP-2 appeared as cell-membrane-associated or in extracellular matrix only in HPDF cell layers. The addition of MMP inhibitor or tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) significantly increased fibrillin-2 accumulation in HPDF cell layers, suggesting that active MMP-2 may degrade fibrillin-2, and that MMPs may play a role in the remodeling of elastic system fibers in periodontal ligament.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (13 results)

All 2007 2005 Other

All Journal Article (10 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Elastic system fibers-Molecular properties of tropoelastin and microfibrils2007

    • Author(s)
      Turuga E, Yajima T
    • Journal Title

      Dentistry in Japan 43

      Pages: 119-122

    • NAID

      10021953561

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fibrillhin-2 degradation by matrix metalloproteinase-2 in periodontium2007

    • Author(s)
      Turuga E, Irie K, Yajima T
    • Journal Title

      Journal of Dental Research 86

      Pages: 352-356

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Elastic system fibers-Molecular properties of tropoelastin and microfibrils-2007

    • Author(s)
      Tsuruga, E., Yajima, T
    • Journal Title

      Dentistry in Japan 43

      Pages: 119-122

    • NAID

      10021953561

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fibrillin-2 degradation by matrix metalloproteinase-2 in periodontium2007

    • Author(s)
      Tsuruga, E., Irie, K., Yajima, T
    • Journal Title

      Journal of Dental Research 86

      Pages: 352-357

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Elastic system fibers -Molecular properties of tropoelastin and microfibrils-2007

    • Author(s)
      Tsuruga E, Yajima T
    • Journal Title

      Dentistry in Japan 43

      Pages: 119-122

    • NAID

      10021953561

    • Related Report
      2007 Annual Research Report 2006 Annual Research Report
  • [Journal Article] Fibrillin-2 degradation by matrix metalloproteinase-2 in periodontium2007

    • Author(s)
      Tsuruga E, Irie K, Yajima T
    • Journal Title

      J Dent Res 86

      Pages: 352-357

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro2005

    • Author(s)
      Turuga E, Yajima T, Irie E
    • Journal Title

      Journal of Biochemistry & Cell Biology 37

      Pages: 120-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro2005

    • Author(s)
      Tsuruga, E., Yajima, T., Irie, K
    • Journal Title

      International Journal of Biochemistry & Cell Biology 37

      Pages: 120-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro.2005

    • Author(s)
      Tsuruga E, Yajima T, Irie K
    • Journal Title

      International Journal of Biochemistry & Cell Biology 37

      Pages: 120-129

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Impaired alveolization in mice deficient in membrane-type matrix metalloproteinase 1 ( MT1-MMP).2005

    • Author(s)
      Irie K, Komori K, Seiki M, Tsuruga E, Sakakura Y, Kaku T, Yajima T
    • Journal Title

      Medical Molecular Morphology 38

      Pages: 43-46

    • NAID

      10018374513

    • Related Report
      2005 Annual Research Report
  • [Presentation] Fibulin-5は微細線維上に存在し、微細線維を形成する2007

    • Author(s)
      敦賀 英知、矢嶋 俊彦、沢 禎彦
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌市
    • Year and Date
      2007-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fubulin-5 are associated with microfibril organizing2007

    • Author(s)
      Tsuruga, E., Yajima, T., Sawa, S
    • Organizer
      Japanese Association for Oral Biology, 49th Annual Meeting
    • Place of Presentation
      Sapporo
    • Year and Date
      2007-08-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.hoku-iryo-u.ac.jp/~anatomy/

    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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