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Analysis of DNAdamage checkpoint regulator gene and cancer control mechanism in oral cancer

Research Project

Project/Area Number 17591923
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionShowa University

Principal Investigator

KOHNO Yohko  Showa University, School of Dentistry, Associate Professor (40195681)

Co-Investigator(Kenkyū-buntansha) TAKIMOTO Masafumi  Showa University, School of Medicine, Associate Professor (40179586)
MURANIATSU Takashi  Tokyo Dental College, Dept. of Pathology, Assistant Professor (00276982)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsOral cancer / DNA damage / p53 / Tumor suppressor gene
Research Abstract

Damage and abnormality of DNA genome is the starting point of the process of malignant transformation of a normal cell. Once DNA damage occurs, eukaryotic cells can activate cell cycle checkpoints, apoptosis or senescence. Some kinases are related to cell cycle checkpoint, apoptosis and senescence pathways, and stimulate and activate other kinases. p53, a transcriptional factor mutated in over 50% of human cancers, is phosphorylated at Ser15 and Ser37 by ATM/ATR and DNA-PK. This phosphorylation promotes the accumulation and functional activation of p53 in response to DNA damage. Active Chk1 and Chk2 can phosphorylate p53 at several serine : sites, enhancing its tetramerization, stability and activity. In 60% of oral cancer, p53 is mutated. It seems that some kinds of oral cancer have different signal transmission pathways which are regarded as conventional DNA damage signal transmission pathways. We used several kinds of oral squamous carcinoma cell lines to check the expression of p53 first. After treatment with genotoxic stress to culture cells, we examined the expression of each protein in DNA damage checkpoint by Western blot analysis, and checked the localization in oral mucosal tissues by immunohistochemistry.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (33 results)

All 2008 2007 2006 2005 Other

All Journal Article (19 results) (of which Peer Reviewed: 4 results) Presentation (14 results)

  • [Journal Article] Heat stress induces ALP activity and HSP25 expression in cultured dental pulp2008

    • Author(s)
      Lee, MK, Muramatsu, T, Uekusa, T, Lee, JH, Shimono, M
    • Journal Title

      Int Endod J 41(2)

      Pages: 158-62

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of connexin43 in cultured dental pulp by antisense oligonucle otides2007

    • Author(s)
      Chung CK.
    • Journal Title

      Cell&Tissue Research 329

      Pages: 295-300

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Osteopontin as biomarker in early invasion by squamous cell carcinoma in tongue2007

    • Author(s)
      Matsuzaki H.
    • Journal Title

      J Oral Pathol Med. 36

      Pages: 30-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inhibition of connexin43 in cultured dental pulp by antisense oligonucleotides2007

    • Author(s)
      Chung, CK, Muramatsu, T, Uekusa, T, Sasaki, H, Shimono, M
    • Journal Title

      Cell & Tissue Research 329(2)

      Pages: 295-300

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Osteopontin as biomarker in early invasion by squamous cell carcinoma in tongue2007

    • Author(s)
      Matsuzaki, H, Shima, K, _Muramatsu, T, Ro, Y, Hashimoto, S, Shibahara, T, Shimono, M
    • Journal Title

      J Oral Pathol Med 36(1)

      Pages: 30-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Osteopontin as biomarker in early invasion by squamous cell carcinoma in tongue2007

    • Author(s)
      Matsuzaki H.
    • Journal Title

      J.Oral Pathol Med. 36・1

      Pages: 30-34

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hypermethylation of CpG islands in p16 as a prognostic factor for diffuse large B-cell lymphoma in a high-risk group2006

    • Author(s)
      Shiozawa, E, Takimoto, M, . Makino, R, Adachi, D, Saito, B, Yamochi-Onizuka, T, Shimozuma, J, Maeda, T, Kohno, Y, Kawakami, K, Nakamaki, T, Tomoyasu, S, Shiokawa, A, Ota, H
    • Journal Title

      Leuk Res

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Connexin 43 transfection in basaloid squamous cell carcinoma cells2006

    • Author(s)
      Shima, K, Muramatsu, T, Abiko, Y, Yamaoka, Y, Sasaki, H, Shimono, M
    • Journal Title

      Oncol Rep 16(2)

      Pages: 285-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Correlation between reduction, of syndecan-1 expression and clinico-pathological parameters in squamous cell carcinoma of tongue2006

    • Author(s)
      Ro, Y, Muramatsu, T, Shima, K, Yajima, Y, Shibahara, T, Noma, H, Shimono, M
    • Journal Title

      Int J Oral Maxillofac Surg 35(3)

      Pages: 252-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Microarray Analysis on Odontogenesis-related -genes in Mouse Dental Papillae2005

    • Author(s)
      Muramatsu, T, Sasaki, H, Yamamoto, H, Kohno, Y, Cho, SW, Jung, FS, Shimono, M
    • Journal Title

      J Hard Tissue Biol 14(2)

      Pages: 180-182

    • NAID

      110005853515

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides2005

    • Author(s)
      Muramatsu, T, Shima, K, Ohta, K, Kizaki, H, Ro, Y, Kohno, Y, Abiko, Y, Shimono, M
    • Journal Title

      Cancer Lett 217(1)

      Pages: 87-95

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] p53 phosphorylation in mouse skin and in vitro human skin model by high-dose-radiation exposure2005

    • Author(s)
      Koike, M, Sugasawa, J, Koike, A, Kohno, Y
    • Journal Title

      J Radiat Res(Tokyo) 46(4)

      Pages: 461-468

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo2005

    • Author(s)
      Kim-Kanayama, J., Suzuki, W., lehikawa., K., Ohki., T., Kohno., Y., Sata., M., Nose., K., S., hibanuma., M., Uni-axial
    • Journal Title

      J Cell Sci 118

      Pages: 937-949

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides2005

    • Author(s)
      Muramatsu, T., Shima, K., Ohta, K., Kizaki, H., Ro, Y., Kohno, Y., Abiko, Y., Shimono, M
    • Journal Title

      Cancer letters 217

      Pages: 87-95

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides2005

    • Author(s)
      Muramatsu T.
    • Journal Title

      Cancer letters 217・1

      Pages: 87-95

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Uni-axial stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo2005

    • Author(s)
      Kim-Kanayama J.
    • Journal Title

      J Cell Sci. 118

      Pages: 937-949

    • Related Report
      2005 Annual Research Report
  • [Journal Article] p-53 phosphorylation in mouse skin and in vitro human skin model by high-dose-radiation exposure2005

    • Author(s)
      Koike M.
    • Journal Title

      J Radiat Res.(Tokyo) 46・4

      Pages: 461-468

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of laminin and integrins in adhesion and migration of junctiona epithelium cells

    • Author(s)
      Kunimatsu T.
    • Journal Title

      J Periodontal Res

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of laminin and integrins in adhesision and migration of junctional epithelium cells

    • Author(s)
      Kinumatsu, T, Hashimoto, S, Muramatsu, T, Sasaki, H, Jung, HS, Yamada, S, Shimono, M
    • Journal Title

      J Periodontal Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 虚空癌のDNA損傷におけるチェックポイント制御機構での各タンパク発現について2007

    • Author(s)
      河野 葉子
    • Organizer
      第96回日本病理学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 口腔癌のDNA損傷におけるチェックポイント制御機構での各タンパク発現について2007

    • Author(s)
      河野 葉子
    • Organizer
      第66回目本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] Analysis of protein expression of DNA damage checkpoint pathway in oral cancer2007

    • Author(s)
      Kohno, Y, Takimoto, M, Shiozawa, E, Ota, H
    • Organizer
      Annual Meeting of the Japanese society of Pathology
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Protein expression of DNA damage checkpoint control pathway in oral cancer2007

    • Author(s)
      Kohno, Y, Takimoto, M, Shiozawa, E, Ota, H
    • Organizer
      Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of DNA damage checkpoint pathway in oral cancer2006

    • Author(s)
      Kohno, Y, Takimoto, M, Shiozawa, E, Ota, H
    • Organizer
      Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] It is not necessary that p53 and H2AX phosphorylation in mouse skin and In Vitro Human skin model by High-Dose-Radiation exposure2006

    • Author(s)
      Sugasawa, J, Kohno, Y, Koike, M
    • Organizer
      Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] p53 Phosphorylation In Mouse Skin. And In Vitro Human Skin Model By High-Dose-Radiation Exposure2006

    • Author(s)
      Kohno, Y., Sugasawa, J., Koike, A., Koike, M
    • Organizer
      7th Joint Meeting of The Histochemical Society & The Japan Society of Histo-chemistry and Cytochemistry
    • Place of Presentation
      Hawaii, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Gluconolactonase activity and deficiency of Senescence Suppressor protein(SMP30)2006

    • Author(s)
      Maruyama, N, Ishigami, A, Handa, S, Kohno, Y
    • Organizer
      Annual Meeting of the Japanese society of Pathology
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Gene expression of tooth bud and pulp by GeneChip microarray2005

    • Author(s)
      Sasaki, H, Muramatsu, T, Ohta, K, Kimizuka, R, Hashimoto, M., Shimono, M
    • Organizer
      Annual meeting of Tokyo dental collage
    • Place of Presentation
      Chiba
    • Year and Date
      2005-06-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Function of syndecan-1 in human oral cancer cell Line2005

    • Author(s)
      Muramatsu, T, Shima, K, Kohno, Y, Abiko, Y, Hashimoto, M, Shimono, M
    • Organizer
      Annual Meeting of the Japanese society of Pathology
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The expression of syndecan-1 in human oral cancer cell line2005

    • Author(s)
      Ro, Y, Shibahara, T, Muramatsu, T, Shima, K, Shimono, M, Ohta, K, Yajima, Y
    • Organizer
      Cngress of Maxillofacial surgery
    • Place of Presentation
      Daemyung Vivaldi park Resort, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of osteopontin in oral squamous cell carcinoma. Oral Oncology Vol.1 No.1 Page1572005

    • Author(s)
      Shima, K, Muramatsu, T, Ro, Y, Sazuka, A, Shimono
    • Organizer
      The 10th International Congress of Oral Cancer
    • Place of Presentation
      Islands of Creta, Greece
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of laminin-5, integrin-beta4 and syndecan-1 in various type of ameloblastoma The Korean Journal of Oral and Maxillofacial Pathology 29(2) : 167, 2005

    • Author(s)
      Sasaki, H, Shima, K, Matsuki, M, Enokiya, Y, Muramatsu, T, Inoue, T, Hashimoto, S, Shimono, M
    • Organizer
      Cogress of Asian Oral and maxillofacial society of Pathology
    • Place of Presentation
      Seoul, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A case of clear cell variant of mucoepidermoid carcinoma with oncocytic metaplasia The Korean Journal of Oral and Maxillofacial Pathology 29(2) : 167, 2005

    • Author(s)
      Muramatsu, T, H., ashimono, S, Inoue, T, Ogawa, I, Takata, T, Shimono, M
    • Organizer
      Cogress of Asian Oral and maxillofacial society of Pathology
    • Place of Presentation
      Seoul, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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