Analysis of DNAdamage checkpoint regulator gene and cancer control mechanism in oral cancer
Project/Area Number |
17591923
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Showa University |
Principal Investigator |
KOHNO Yohko Showa University, School of Dentistry, Associate Professor (40195681)
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Co-Investigator(Kenkyū-buntansha) |
TAKIMOTO Masafumi Showa University, School of Medicine, Associate Professor (40179586)
MURANIATSU Takashi Tokyo Dental College, Dept. of Pathology, Assistant Professor (00276982)
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Project Period (FY) |
2005 – 2007
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Project Status |
Completed (Fiscal Year 2007)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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Keywords | Oral cancer / DNA damage / p53 / Tumor suppressor gene |
Research Abstract |
Damage and abnormality of DNA genome is the starting point of the process of malignant transformation of a normal cell. Once DNA damage occurs, eukaryotic cells can activate cell cycle checkpoints, apoptosis or senescence. Some kinases are related to cell cycle checkpoint, apoptosis and senescence pathways, and stimulate and activate other kinases. p53, a transcriptional factor mutated in over 50% of human cancers, is phosphorylated at Ser15 and Ser37 by ATM/ATR and DNA-PK. This phosphorylation promotes the accumulation and functional activation of p53 in response to DNA damage. Active Chk1 and Chk2 can phosphorylate p53 at several serine : sites, enhancing its tetramerization, stability and activity. In 60% of oral cancer, p53 is mutated. It seems that some kinds of oral cancer have different signal transmission pathways which are regarded as conventional DNA damage signal transmission pathways. We used several kinds of oral squamous carcinoma cell lines to check the expression of p53 first. After treatment with genotoxic stress to culture cells, we examined the expression of each protein in DNA damage checkpoint by Western blot analysis, and checked the localization in oral mucosal tissues by immunohistochemistry.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Osteopontin as biomarker in early invasion by squamous cell carcinoma in tongue2007
Author(s)
Matsuzaki, H, Shima, K, _Muramatsu, T, Ro, Y, Hashimoto, S, Shibahara, T, Shimono, M
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Journal Title
J Oral Pathol Med 36(1)
Pages: 30-4
Description
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[Journal Article] Hypermethylation of CpG islands in p16 as a prognostic factor for diffuse large B-cell lymphoma in a high-risk group2006
Author(s)
Shiozawa, E, Takimoto, M, . Makino, R, Adachi, D, Saito, B, Yamochi-Onizuka, T, Shimozuma, J, Maeda, T, Kohno, Y, Kawakami, K, Nakamaki, T, Tomoyasu, S, Shiokawa, A, Ota, H
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[Journal Article] Connexin 43 transfection in basaloid squamous cell carcinoma cells2006
Author(s)
Shima, K, Muramatsu, T, Abiko, Y, Yamaoka, Y, Sasaki, H, Shimono, M
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Journal Title
Oncol Rep 16(2)
Pages: 285-8
Description
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[Journal Article] Correlation between reduction, of syndecan-1 expression and clinico-pathological parameters in squamous cell carcinoma of tongue2006
Author(s)
Ro, Y, Muramatsu, T, Shima, K, Yajima, Y, Shibahara, T, Noma, H, Shimono, M
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Journal Title
Int J Oral Maxillofac Surg 35(3)
Pages: 252-7
Description
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[Journal Article] Microarray Analysis on Odontogenesis-related -genes in Mouse Dental Papillae2005
Author(s)
Muramatsu, T, Sasaki, H, Yamamoto, H, Kohno, Y, Cho, SW, Jung, FS, Shimono, M
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Journal Title
J Hard Tissue Biol 14(2)
Pages: 180-182
NAID
Description
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[Journal Article] Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides2005
Author(s)
Muramatsu, T, Shima, K, Ohta, K, Kizaki, H, Ro, Y, Kohno, Y, Abiko, Y, Shimono, M
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Journal Title
Cancer Lett 217(1)
Pages: 87-95
Description
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[Journal Article] stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo2005
Author(s)
Kim-Kanayama, J., Suzuki, W., lehikawa., K., Ohki., T., Kohno., Y., Sata., M., Nose., K., S., hibanuma., M., Uni-axial
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Journal Title
J Cell Sci 118
Pages: 937-949
Description
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[Journal Article] Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides2005
Author(s)
Muramatsu, T., Shima, K., Ohta, K., Kizaki, H., Ro, Y., Kohno, Y., Abiko, Y., Shimono, M
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Journal Title
Cancer letters 217
Pages: 87-95
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[Journal Article] Involvement of laminin and integrins in adhesision and migration of junctional epithelium cells
Author(s)
Kinumatsu, T, Hashimoto, S, Muramatsu, T, Sasaki, H, Jung, HS, Yamada, S, Shimono, M
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Journal Title
J Periodontal Res (in press)
Description
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[Presentation] Gene expression of tooth bud and pulp by GeneChip microarray2005
Author(s)
Sasaki, H, Muramatsu, T, Ohta, K, Kimizuka, R, Hashimoto, M., Shimono, M
Organizer
Annual meeting of Tokyo dental collage
Place of Presentation
Chiba
Year and Date
2005-06-04
Description
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[Presentation] Function of syndecan-1 in human oral cancer cell Line2005
Author(s)
Muramatsu, T, Shima, K, Kohno, Y, Abiko, Y, Hashimoto, M, Shimono, M
Organizer
Annual Meeting of the Japanese society of Pathology
Place of Presentation
Yokohama
Description
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[Presentation] The expression of syndecan-1 in human oral cancer cell line2005
Author(s)
Ro, Y, Shibahara, T, Muramatsu, T, Shima, K, Shimono, M, Ohta, K, Yajima, Y
Organizer
Cngress of Maxillofacial surgery
Place of Presentation
Daemyung Vivaldi park Resort, Korea
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[Presentation] Expression of laminin-5, integrin-beta4 and syndecan-1 in various type of ameloblastoma The Korean Journal of Oral and Maxillofacial Pathology 29(2) : 167, 2005
Author(s)
Sasaki, H, Shima, K, Matsuki, M, Enokiya, Y, Muramatsu, T, Inoue, T, Hashimoto, S, Shimono, M
Organizer
Cogress of Asian Oral and maxillofacial society of Pathology
Place of Presentation
Seoul, Korea
Description
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[Presentation] A case of clear cell variant of mucoepidermoid carcinoma with oncocytic metaplasia The Korean Journal of Oral and Maxillofacial Pathology 29(2) : 167, 2005
Author(s)
Muramatsu, T, H., ashimono, S, Inoue, T, Ogawa, I, Takata, T, Shimono, M
Organizer
Cogress of Asian Oral and maxillofacial society of Pathology
Place of Presentation
Seoul, Korea
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