DEVELOPMENT OF CULTURE METHODS FOR EXOCRINE GLAND STEM CELLS USING GENE-TRANSDUCED FEEDER CELLS AND THEIR APPLICATION FOR TREATMENT
| Project/Area Number |
17591930
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
MISHIMA Kenji TSURUMI UNIVERSITY, DENTISTRY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (50275343)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Ichiro TSURUMI UNIVERSITY, DENTISTRY, PROFESSOR, 歯学部, 教授 (60147634)
INOUE Hiroko TSURUMI UNIVERSITY, DENTISTRY, ASSISTANT PROFESSOR, 歯学部, 講師 (50367306)
TSUBOTA Kazuo KEIO UNIVERSITY, MEDICINE, PROFESSOR, 医学部, 教授 (40163878)
山田 耕一 鶴見大学, 歯学部, 助手 (60367307)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | SALIVARY GLANDS / REGENERATIVE MEDICINE / HYPOFUNCTION / SP CELLS |
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| Research Abstract |
Stem cell-based therapy has been proposed as a promising strategy for regenerating tissues lost through incurable diseases. Although the technology to purify stem cells largely depends on specific surface markers, side population (SP) cells are applicable without stem cell markers. However, the functions of SP cells still remain controversial. To explore the precise functions of SP cells, we assessed their functions in mouse exocrine glands, namely, salivary glands, because these glands serve as convenient models whose ability can be easily evaluated by measurement of their tear and saliva secretions, respectively. Here we show that SP cell transplantation restores the functions of both the glands rendered hypofunctional by irradiation, and clarify a putative substrate that protects the cells from damage by oxidative stress. We isolated SP cells from the lachrymal and salivary glands and transplanted them into mice with irradiation-induced hypofunction in each gland. The results revealed that the secretions from both glands in the recipient mice were restored within two months after the transplantation, although transplanted cells were only sparsely distributed and produced no outgrowths, indicating that soluble factors secreted by SP cells, and not reconstituted cells, possibly restored the functions of the residual glands. Clusterin, which has been identified as one of the SP cell-specific genes, is a secretory glycoprotein, and been shown to inhibit cell death and reactive oxygen species (ROS) accumulation induced by oxidative stress. Our results indicate that the benefits of SP cell transplantation are attributable to not cell transplantation-mediated reconstitution of the glands but the functions of SP cell-derived soluble factors including clusterin.
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Report
(3 results)
Research Products
(4 results)
