Basic study about cell-based therapy by cell life span control using human papillomavirus

• Project/Area Number: 17591931
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: TERAI Masanori National Research Institute for Child Health and Development, 生殖医療研究部, 共同研究員 (70359917)
• Co-Investigator(Kenkyū-buntansha): UMEZAWA Akihiro 国立成育医療センター(研究所), 生殖医療研究部, 部長 (70213486) ; HATA Junichi 国立成育医療センター, 名誉総長 (90051614)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,000,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 再生医学 / 細胞・組織 / ウイルス

Research Abstract

臍帯血からの間葉系細胞のstrainの樹立さらに寿命を延長することに成功し、それぞれの細胞の特徴性格づけも行った。さらに月経血からの間葉系細胞の骨格筋への高率な分化誘導にも成功した。この技術はヒト正常体細胞を用いた将来の細胞移植治療の応用の際に大きな利益をもたらすと考える。

Research Products

• [Journal Article] 臍帯血由来間葉系幹細胞を利用した再生医療 (2008)
  • Author(s): 寺井政憲
  • Journal Title: 医学のあゆみ 226 Pages: 187-187
  • Peer Reviewed
• [Journal Article] A role of Wnt/{beta}-catenin signals in hepatic fate specification of human umbilical cord blood-derived mesenchymal stem cells. (2007)
  • Author(s): Yoshida Y, Shimomura T, Sakabe T, Ishii K, Gonda K, Matsuoka S, Watanabe Y, Takubo K, Tsuchiya H, Hoshikawa Y, Kurimasa A, Hisatome I, Uyama T, Terai M, Umezawa A, Shiota G.
  • Journal Title: American Journal of Physiology Gastrointestinal and Liver Physiology 293 Pages: 1089-1098
  • Peer Reviewed
• [Journal Article] The Significant Cardiomyogenic Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Vitro. (2007)
  • Author(s): Nishiyama N, Miyoshi S, Hida N, Uyama T, Okamoto K, Ikegami Y, Miyado K, Segawa K, Terai M, Sakamoto M, Ogawa S, Umezawa A.
  • Journal Title: Stem Cells 25 Pages: 2017-2024
  • Peer Reviewed
• [Journal Article] Menstrual blood-derived cells confer human dystrophin expression in the murine model of duchenne muscular dystrophy via cell fusion and myogenic transdifferentiation. (2007)
  • Author(s): Cui CH, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, Umezawa A.
  • Journal Title: Molecular Biology of the Cell 18 Pages: 1586-1594
  • Peer Reviewed
• [Journal Article] Chromosomal instability in human mesenchymal stem cells Immortalized with human papilloma yirus E6, E7, and hTERT genes (2007)
  • Author(s): Takeuchi M, Takeuchi K, Kohara A, Satoh M, Shioda S, Ozawa Y, OhtaniA, Morita K, Hirano T, Terai M, Umezawa A, Mizusawa H.
  • Journal Title: In Vitro Cellular&Developmental Biology-Animal 43巻3-4号 Pages: 129-138
• [Journal Article] Menstrual blood-derived cells confer human dystrophin expression in the murine model of duchenne muscular dystrophy via cell fusion and myogenic transdifferentiation (2007)
  • Author(s): Cui CH, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, Umezawa A.
  • Journal Title: Molecular Biology of the Cell 18巻5号 Pages: 1586-1594
• [Journal Article] The Significant Cardiomyogenic Potential of Human Umbilical CordBlood-Derived Mesenchymal Stem Cells in Vitro (2007)
  • Author(s): Nishiyama N, Miyoshi S, Hida N, Uyama T, Okamoto K, Ikegami Y, Miyado K, Segawa K, Terai M, Sakamoto M, Ogawa S, Umezawa A.
  • Journal Title: Stem Cells 25巻8号 Pages: 2017-2024
• [Journal Article] A role of Wnt/{beta}-catenin signals in hepatic fate specification of human umbilical cord blood-derived mesenchymal stem cells (2007)
  • Author(s): Yoshida Y, Shimomura T, Sakabe T, Ishii K, Gonda K, Matsuoka S, Watanabe Y, Takubo K, Tsuchiya H, Hoshikawa Y, Kurimasa A, Hisatome I, Uyama T, Terai M, Umezawa A, Shiota G.
  • Journal Title: American Journal of Physiology Gastrointestinal and Liver Physiology 293巻5号 Pages: 1089-1098
• [Journal Article] Diversifying selection in human papillomavirus type 16 (HPV16) lineages based on complete genome analysis. (2005)
  • Author(s): Chen Z, Terai M, Fu L, Herrero R, DeSalle R, Burk RD.
  • Journal Title: Journal of Virology 79 Pages: 7014-7023
  • Peer Reviewed
• [Journal Article] Overlapping reading frames in closely related human papillomaviruses results in modular rates of selection within E2. (2005)
  • Author(s): Narechania A, Terai M, Burk RD.
  • Journal Title: Journal of General Virology 86 Pages: 1307-1313
  • Peer Reviewed
• [Journal Article] Immortalization of human fetal cells : The life span of umbilical-cord-blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway. (2005)
  • Author(s): Terai M, Uyama T, Sugiki T,Li XK, Umezawa A, Kiyono T.
  • Journal Title: Molecular Biology of the Cell 16 Pages: 1491-1499
  • Peer Reviewed
• [Journal Article] Improvement of musculoskeletal function by cell-based therapy using mesenchymal stem cells with a prolonged life span. (2005)
  • Author(s): Umezawa A, Terai M.
  • Journal Title: Clinical Calcium 15 Pages: 805-812
• [Journal Article] いぼの分子、細胞生物学 (2005)
  • Author(s): 寺井政憲, 清野 透
  • Journal Title: Monthly Book Derma 97 Pages: 30-37
• [Journal Article] Diversifying selection in human papillomavirus type 16 lineages based on complete genome analyses. (2005)
  • Author(s): Chen Z, et al.
  • Journal Title: Journal of Virology 79・11 Pages: 7014-7023
• [Journal Article] Improvement of musculoskeletal function by cell-based therapy using mesenchymal stem cell with a prolonged life span. (2005)
  • Author(s): Umezawa A, et al.
  • Journal Title: Clinical Calcium 15・5 Pages: 805-812
• [Journal Article] Overlapping reading frames in closely related human papillomaviruses result in modular rates of selection within E2. (2005)
  • Author(s): Narechania A, et al.
  • Journal Title: Journal of General Virology 86・5 Pages: 1307-1313
• [Journal Article] Immortalization of human fetal cells : the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway. (2005)
  • Author(s): Terai M, et al.
  • Journal Title: Molecular Biology of the Cell 16・3 Pages: 1491-1499
• [Journal Article] いぼの分子、細胞生物学 (2005)
  • Author(s): 寺井政憲, 他
  • Journal Title: Monthly Book Derma 97・2 Pages: 30-37
• [Presentation] Immortalization of human fetal cells : The life span of umbilical-cord-blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway. Joint Congress of "the 45th Annual Meeting of the Japanese Society for Artificial Organs" and "the 2nd Meeting of the International Federation for Artificial Organs" (2007)
  • Author(s): Terai M.
  • Organizer: Symposium「Tissue Engineering」
  • Place of Presentation: 大阪
  • Year and Date: 2007-10-28