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Expression of OCZF directed by the cathepsin K promoter affects bone mass and osteoclast formation in Transgenic Mice.

Research Project

Project/Area Number 17591941
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionSaga University

Principal Investigator

KUKITA Akiko  Saga University, Medicine, Associate Professor, 医学部, 助教授 (30153266)

Co-Investigator(Kenkyū-buntansha) KUKITA Toshio  Kyushu University, Dentistry, Professor, 大学院・歯学研究院, 教授 (70150464)
SHOBUIKE Takeo  Saga University, Medicine, Assistant Professor, 医学部, 助手 (70336113)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsosteoclast / transcriptional factor / transgenic mouse / 転写制御因子 / カテプシンK
Research Abstract

The OCZF gene product is a member of POK protein family that contains two domains of POZ/BTB and zinc finger in their N-and C-terminal region respectively, and that is shown to be involved in various cellular processes. Suppression of OCZF by antisense RNA inhibits osteoclast differentiation in vitro, indicating that OCZF is involved in osteoclastogenesis, but in vivo role in the process is not clear. To this end we generated transgenic mice, which expressed OCZF under the control of mouse cathepsin K promoter (Ctsk-OCZF). Nine transgenic mice were identified among the offspring by PCR and Southern blot analysis of mouse tail genomic DNA. Transgene expression was verified by RT-PCR using osteoclasts differentiated in vitro from bone marrow at 10 weeks of age. Two lines of the transgenic animals exhibited relatively high transgene expression, and were further investigated. Micro-CT and peripheral quantitative CT (pQCT) analyses and histomorphometry of femur were performed in mice at 6 weeks of age. Micro-CT imaging demonstrates a reduction in trabecular bone volume in the transgenic mice relative to normal littermates. pQCT analyses also demonstrate a reduction in bone mineral density and bone mineral content especially in trabecular bone with a decrease in strength strain index, whereas those in cortical bone were slightly affected. Consistently, von Kossa staining shows a smaller mineralized area in the transgenic mice than in normal littermates. Histological analyses with tartrate-resistant acid phosphatase staining and calcein labeling indicate an increase in the number of osteoclast, whereas bone formation rate did not significantly change. These data suggest that OCZF/LRF has an important role in osteoclastogenesis in vivo.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (16 results)

All 2007 2006 2005

All Journal Article (16 results)

  • [Journal Article] Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans.2007

    • Author(s)
      Yamaguchi N
    • Journal Title

      FEMS Immunol Med Microbiol. 49(1)

      Pages: 28-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FGF23 induces expression of two isoforms of Nabs which are corepressor of Egr-1.2007

    • Author(s)
      Fukuda T
    • Journal Title

      Biochem. Biophys. Res. Comm. 353

      Pages: 147-151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FGF23 induces expression of two isoforms of NAB2, which are corepressors of Egr-12007

    • Author(s)
      Fukuda T.
    • Journal Title

      Biochem.Biophys.Res.Comm. 353

      Pages: 147-147

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans.2007

    • Author(s)
      Yamaguchi N.
    • Journal Title

      FEMS Immunology & Microbiology 49

      Pages: 28-28

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Regulation of osteoclastogenesis by Simon extracts composed of caffeic acid and related compounds : succesful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats.2006

    • Author(s)
      Tang QY
    • Journal Title

      Histochem Cell Biol. 125(3)

      Pages: 215-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The contribution of platelet-derived growth factor, transforming growth factor-betal, and insulin-like growth factor-I in platelet-rich plasma to the proliferation of osteoblast-like cells.2006

    • Author(s)
      Ogino Y
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(6)

      Pages: 724-729

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] マンネンタケ(Genoderma lucidum)エタノール抽出物による骨粗鬆症予防効果2006

    • Author(s)
      清水邦義
    • Journal Title

      機能性食品と薬理栄養 日本機能性食品医用学会誌 4(1)

      Pages: 29-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of osteoclastogenesis by Simon extracts composed of caffeic acid and related compounds : successful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats.2006

    • Author(s)
      Tang QY
    • Journal Title

      Histochem Cell Biol. 125(3)

      Pages: 215-225

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] The contribution of platelet-derived growth factor, transforming growth factor-beta1, and insulin-like growth factor-I in platelet-rich plasma to the proliferation of osteoblast-like cells.2006

    • Author(s)
      Ogino Y
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(6)

      Pages: 724-729

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prevention effect of bone loss in calcium deficient ovariectomized rats by the ethanol extract of Ganoderma lucidum2006

    • Author(s)
      Shimizu K.
    • Journal Title

      Associate Journal of Japanese Society for Medical Use of Functional Foods 4(1)

      Pages: 29-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] マンネンタケ(Genoderme lucidum)エタノール抽出物による骨粗鬆症予防効果2006

    • Author(s)
      清水邦義
    • Journal Title

      機能性食品と薬理栄養 4

      Pages: 29-29

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The contribution of platelet-derived growth factor, transforming growth factor-<beta>1 and insulin-like growth factor-1 in platelet-rich plasma (PRP) to the proliferation of osteoclast- like cells2006

    • Author(s)
      Ogino Y.
    • Journal Title

      Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 101

      Pages: 724-724

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of genes differentially expressed in osteoclast-like cells.2005

    • Author(s)
      Nomiyama H
    • Journal Title

      J Interferon Cytokine Res. 25(4)

      Pages: 227-231

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Ameloblastoma induces osteoclastogenesis : a possible role of ameloblastoma in expanding in the bone.2005

    • Author(s)
      Sandra F
    • Journal Title

      Oral Oncol. 41(6)

      Pages: 637-644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Inhibition of histone deacetylase suppresses osteoclastogenesis and bone destruction by inducing IFN-beta production.2005

    • Author(s)
      Nakamura T
    • Journal Title

      J Immunol. 175(9)

      Pages: 5809-5816

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Inhibition of histone deacetylase suppresses osteoclastogenesis and bone destruction by inducing IFN-beta production.2005

    • Author(s)
      Nakamura T
    • Journal Title

      J Immunol. 175 (9)

      Pages: 5809-5816

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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