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Role of neuron-glial cross talk in the trigeminal ganglia to hyperalgesia

Research Project

Project/Area Number 17591953
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

TAKEDA Mamoru  The Nippon Dental University, School of Life Dentistry at Tokyo, Associate Professor (20227036)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,550,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsTrigeminal ganglion / Glia / Hyperalciesia / Interleukin-1β / Perforated patch-clamp / Immunohistochemistry / Microiontophoresis / Extracellular recording / Interleukin-1β / 炎症 / マルチバレル / 微小電気泳動 / 免疫組織化学
Research Abstract

The aim of the present study was to investigate whether neuron-glial cross talk in the trigeminal ganglia contribute to modulation of neuronal excitability of nociceptive trigeminal ganglion neurons during peripheral inflammation. Complete freund's adjuvant (CFA) was injected into rat facial skin to produce inflammation. The threshold for escape from mechanical stimulation applied to the whisker pad in inflamed rats was significantly lower than that in control. Two days after CFA injection, the mean percentage of TRG neurons encircled by glial fibrillary acidic protein (GFAP)/IL-1βimmunoreactivities were coexpressed in the same cells. Fluorogold labeling identified the site of inflammation. The number of FG-labeled IL-1 receptor type I (IL-1RI) small TRG neurons in inflamed rats was significantly greater than thai in control. In the FG labeled small TRG neurons, the size of IL-1β induced-depolarization in inflamed rats was larger than in control. Aσ-nociceptive TRG neurons responding to electrical stimulation of the whisker pad was recorded in pentobarbital-anesthetized rats. The number of Aσ-TRG neurons with spontaneous firings and their firing rate in inflamed rats were significantly larger than in control rats. The firing rates of the Aσ-TRG neuronal spontaneous activity were current-dependently decreased by local iontophoretic application of an interleukin I receptor type I antagonist (IL-1ra) in inflamed rats. The mechanical threshold of nociceptive-TRG neurons in inflamed rats was significantly lower than that in control rats, but was not significantly different between control rats and inflamed rats after iontophoretic application of an IL-1ra. These results suggest that under inflammation modulates the excitability of nociceptive Aσ-TRG neurons innervating the facial skin via IL-1β paracrine action within trigeminal ganglia. Such an IL-1β release could be important in determining trigeminal inflammatory hyperalgesia.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (21 results)

All 2008 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (10 results) Book (2 results)

  • [Journal Article] Contribution activated interleukin receptors in trigeminal ganglion neurons to hyperalgesia via satellite glial Interleukin-1β paracrine mechanism2008

    • Author(s)
      Takeda M, Takahashi M, Matsumoto S
    • Journal Title

      Brain Behavior Immunity (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Contribution activated interleukin receptors in trigeminal ganglion neurons to hyperalgesia via satellite glial interleukin-1β paracrine mechanism2008

    • Author(s)
      Takeda, M., Takahashi, M., Matsumoto, S
    • Journal Title

      Brain Behavior Immunity (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Modulation of neuronal signal by cross-talk within sensory ganglia : contribution to pathological pain2008

    • Author(s)
      Takeda, M., Matsumoto, S
    • Journal Title

      Neuronal Pathways Research(In : Editor, Florian L Pichler, New York, USA, Nova Publishers) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Contribution activated interleukin receptors in trigeminal ganglion neurons to hyperalgesia via satellite glial Interleukin-1β paracrine mechanism.2008

    • Author(s)
      Takeda M, Takahashi M, Matsumoto S.
    • Journal Title

      Brain Behavior Immunity (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced excitability of noaceptive trigeminal ganglion neurons by satellite glial cytokine following peripheral inflammation2007

    • Author(s)
      Takeda M, Tanimto T, Kadoi J, Takahashi M, Nasu M, Kitagawa J, Matsumoto S.
    • Journal Title

      Pain 129

      Pages: 155-166

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Enhanced excitability of nociceptive trigeminal ganglion neurons by satellite glial cytokine following peripheral inflammation.2007

    • Author(s)
      Takeda, M., Tanimoto, T., Kadoi, J., Takahashi, M., Nasu, M., Kitagawa, J., Matsumoto, S
    • Journal Title

      Pain 129

      Pages: 155-166

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Enhanced excitability of nociceptive trigeminal ganglion neurons by satellite glial cytokine following peripheral inflammation2007

    • Author(s)
      Takeda M, Tanimoto T, Kadoi J, Takahashi M, Nasu M, Kitagawa J, Mastsumoto S.
    • Journal Title

      Pain 129

      Pages: 155-166

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced excitability of nociceptive trigeminal ganglion neurons by satellite glial cytokine following peripheral inflammation2007

    • Author(s)
      M.Takeda et al.
    • Journal Title

      Pain (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Interleukin-1β enhanced excitability of trigeminal root ganglion neurons via activation of satellite glia following inflammation2006

    • Author(s)
      M.Takeda et al.
    • Journal Title

      Neuroscience Research 55

    • Related Report
      2006 Annual Research Report
  • [Presentation] Interleuki-1β enhanoed excitability of trigeminal root ganglion neurons via activation of satellite glia following inflammation2007

    • Author(s)
      Takeda M, Takahashi M, Matsumoto S.
    • Organizer
      第30回日本神軽科学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Activation of interleukin receptor of nociceptive trigeminal ganglion neurons via satellite glia IL-1β contributes to the inflammatory hyperalgesia following peripheral inflammation2007

    • Author(s)
      Takeda, M., Takahashi, M., Matsumoto, S
    • Organizer
      The 30th annual meeting of Japan Neuroscience Society(Neurosci Res 58(Supple) S125,2007)
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interleukin-1β enhanced excitability of trigeminal root ganglion neurons viaactivation of satellite glia following inflammation.2007

    • Author(s)
      Takeda M, Kadoi J, Takahashi M, Nasu M, Matsumoto S.
    • Organizer
      第30回 日本神経科学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-09-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Role of neuron-glial cross Talk in the trigeminal ganglia to hyperalgesia2007

    • Author(s)
      Takeda, M., Takahashi, M., Matsumoto, S
    • Organizer
      The 49th Annual meeting of Japanease Association for Oral Biology(J Oral Biosci 49 (Supple) 171,2007)
    • Year and Date
      2007-08-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 三叉神経節内におけるニューロングリア細胞間クロストークの痛覚過敏に対する役割2007

    • Author(s)
      武田 守、高橋誠之、松本茂二
    • Organizer
      第49回 歯科基礎医学学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] Peripheral inflammation enhanced excitabillity of nociceptive trigeminal ganglion neurons by satellite glial cytokine2007

    • Author(s)
      Takeda M, Tanimoto T, Kadoi J, Takahashi M, Nasu M, Kitagawa J, Matsumoto S.
    • Organizer
      第84回日本生理学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peripheral inflammation enhanced excitability of nociceptive trigeminal ganglion neurons by satellite glial cytokine2007

    • Author(s)
      Takeda, M., Tanimoto, T., Kadoi, J., Takahashi, M., Nasu, Kitagawa, J., Mastumoto, S
    • Organizer
      The 84th annual meeting of Physiological Society of Japan(J Physiol Sci 57(Supple),S111,2007)
    • Year and Date
      2007-03-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 三叉神経節内におけるニューロングリア細胞間クロストークの痛覚過敏に対する役割2007

    • Author(s)
      武田 守、高橋 誠之、松本 茂二
    • Organizer
      第49回歯科基礎医学学術大会
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interleukin-1β enhanced excitability of trigeminal root ganglion neurons via activation of satellite glia following inflammation2006

    • Author(s)
      Takeda M, Kadoi J, Takahashi M, Nasu M, Matsumoto S.
    • Organizer
      第29回日本神経科学会大会
    • Place of Presentation
      京都
    • Year and Date
      2006-07-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interleukin-1β enhanced excitability of trigeminal root ganglion neurons via activation of satellite glia following inflammation.2006

    • Author(s)
      Takeda, M, Kadoi, J, Takahashi, M, Nasu, M, Matsumoto, S
    • Organizer
      The 29th annual meeting of Japan Neuroscience Society(Neurosci Res 55(Supple),S187,2006)
    • Year and Date
      2006-07-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Modulation of neuronal signal by cross-talk within sensory ganglia:contribution to pathological pain, In:Neuronal Pathway Research(Eds.FL.Pichler)2008

    • Author(s)
      M. Takeda, S. Matsumoto
    • Publisher
      Nova Publishers.New York,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Modulation of neuronal signal by cross-talk within sensory ganglia: contribution to pathological pain, In: Neuronal Pathway Research(Eds. FL. Pichler)2008

    • Author(s)
      M. Takeda and S. Matsumoto
    • Publisher
      Nova Publishers. New York, USA, (In press)
    • Related Report
      2007 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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