The research of the generation and mechanism for the lesion of the ulcer and erosion in oral mucosa
| Project/Area Number |
17591959
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tohoku University |
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Principal Investigator |
SUGAWARA Yumiko Tohoku University, Tohoku University, Hospital, Assistant Professor (30235866)
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| Co-Investigator(Kenkyū-buntansha) |
SHOUJI Noriaki Hospital, 病院, Assistant Professor (70250800)
SUGAWARA Shunji Graduate School of Dentistry, 大学院・歯学研究科, Proffessor (10241639)
SASANO Takashi Graduate School of Dentistry, 大学院・歯学研究科, Proffessor (10125560)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥2,950,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | oral mucosa / The innate immune system / IL-18 / the lesion of the ulcer and erosion / TLRs / NODs / IL-17 / Fas / FasL |
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| Research Abstract |
Oral epithelial cells may form the first barriers of defense against oral bacteria in periodontal tissue. In this study, we thoroughly examined the expression of Toll-like receptor (TLR)2, TLR4, nucleotide-binding oligomerization domain (NOD)l and NOD2, and their possible antibacterial roles in terms of the expression of peptidoglycan recognition proteins (PGRPs) and b-defensin 2 in oral epithelial cells.
1) Oral epithelial cells constitutively express TLR2, TLR4, NOD1 and NOD2 molecules.
2) Oral epithelial cells do not secrete proinflammatory cytokines upon stimulation with synthetic PAMPs.
3) Synthetic PAMPs stimulated human oral epithelial cells to increase the expressions of four lands of PGRPs and b-defensin 2.
4) Up-regulation of PGRPs by Pam3CSSNA, lipid A, iE-DAP, and AMP were performed through TLR2, TLR4, NODI and NOD2, respectively.
5) The up-regulation of PGRPs induced by synthetic PAMPs was significantly inhibited in NF-kB p50- and p65-silenced cells.
6) Inflamed gingival epithelial cells clearly express PGRP proteins.
7) meso-DAP itself activates epithelial cells but not monocytic cells through NOD1.
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Report
(4 results)
Research Products
(132 results)
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[Journal Article] Expression of various Toll-Like receptors, NOD, and NOD2, in human oral epithelial cells, and their function.2008
Author(s)
Yumiko Sugawara, Akiko Uehara, Yukari Fujimoto, Shoichi hKusumoto, , Koichi Fukase, Takashi Sasano, Haruhiko Takada.
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Journal Title
Interface Oral Health Science 2007 2
Pages: 225-230
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Human parotid saliva contains soluble toll-like receptor (TLR) 2 and modulates TLR2-mediated interleukin-8 production by monocytic cells.2007
Author(s)
Kuroishi, T, Tanaka, Y, Sakai, A, Sugawara, Y, Komine, K, Sugawara,S
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Journal Title
Mol Immunol 44(8)
Pages: 1969-1979
Related Report
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[Journal Article] Toll-like receptors, NOD1, and NOD2 in oral epithelial cells.2006
Author(s)
Sugawara, Y. Uehara, A. Fujimoto, Y. Kusumoto, S. Fukase, K. Shibata, K. Sugawara, S. Sasano, T. Takada, H.
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Journal Title
J Dent Res 85
Pages: 524-529
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Toll-like receptors, NOD1, and N0D2 in oral epithelial cells2006
Author(s)
Sugawara, Y. Uehara, A. Fujimoto, Y. Kusumoto. S. Fukase, K. Shibata, K. Sugawara, S. Sasano, T. Takada, H.
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Journal Title
J Dent Res 85 (6)
Pages: 524-529
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Toll-like receptors, N0D1, and N0D2 in oral epithelial cells.2006
Author(s)
Sugawara, Y, Uehara, A, Fujimoto, Y, Kusumoto, S, Fukase, K, Shibata, K, Sugawara, S, Sasano, T, Takada,H
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Journal Title
J Dent Res 85(6)
Pages: 524-529
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[Journal Article] Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins viatoll-like receptors, NOD1 and NOD2 in human oral epithelial cells.2005
Author(s)
Uehara A, Sugawara Y, Kurata S, Fujimoto Y, Fukase K, Kusumoto S, Satta Y, Sasano T, Sugawara S, Takada H.
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Journal Title
Cellular Microbiology 7
Pages: 675-686
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Involvement of neutrophil recruitment and protease-activated receptor 2 activation in induction of IL-18 in mice.2005
Author(s)
Ikawa K, Nishioka T, Yu Z, Sugawara Y, Kawagoe J, Takizawa T, Primo V, Nikolic B, Kuroishi T, Sasano T, Shimauchi H, Takada H, Endo Y and Sugawara S.
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Journal Title
J Leukoc Biol 7
Pages: 1118-1126
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins via toll-like receptors , NOD1 and N0D2 in human oral epithelial cells2005
Author(s)
Uehara A, Sugawara Y, Kurata S, Fujimoto Y, Fukase K, Kusumoto S, Satta Y, Sasano T, Sugawara S, Takada H.
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Journal Title
Cellular Microbiology 7 (5)
Pages: 675-686
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Involvement of neutrophil recruitment and protease-activated receptor 2 activation in induction of IL-18 in mice2005
Author(s)
Ikawa K, Nishioksi T, Yu Z, Sugawara Y, Kawagoe J, TaMzawa T, Prirao V, Nikolic B, Kuroishi T, Sasano T, Shimauchi H, Takada H, Endo Y and Sugawara S.
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Journal Title
J Leukoc Biol 7
Pages: 1118-1126
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins via toll-like receptors, NOD1 and NOD2 in human oral epithelial cells2005
Author(s)
Uehara A, Sugawara Y, Kurata S, Fujimoto Y, Fukase K, Kusumoto S, Satta Y, Sasano T, Sugawara S, Takada H
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Journal Title
Cellular Microbiology 7(5)
Pages: 675-686
Related Report
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[Journal Article] Involvement of neutrophil recruitment and protease-activated receptor 2 activation in induction of IL-18 in mice2005
Author(s)
Ikawa K, Nishioka T, Yu Z, Sugawara Y, Kawagoe J, Takizawa T, Primo V, Nikolic B, Kuroishi T, Sasano T, Shimauchi H, Takada H, Endo Y, Sugawara S
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Journal Title
J Leukoc Biol 7
Pages: 1118-1126
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[Presentation] A case of Nasal NK/T cell lymphoma2006
Author(s)
Sugawara Y, Sato S, Sasano T.
Organizer
The 19^<th> Annual Meeting of the Japanease Society of Oral Diagnosis/Oral Medicine
Place of Presentation
Kohriyama, Japan
Year and Date
2006-04-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Involvement of neutrophils and protease-activated receptor-2 (PAR-2) in the induction of IL-18 from mucosal cells in mice.2005
Author(s)
Nishioka T, Ikawa K, Yu Z, Sugawara Y, Kawagoe J, Takizawa T, Primo V, Nikolic B, Sasano T, Shimauchi H, Takada H, Endo Y and Sugawara S.
Organizer
12th International Congress of Mucosal Immunology.
Place of Presentation
Boston
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Involvement of neutrophils and protease-activated receptor-2 (PAR-2) in the induction of IL-18 from mucosal cells in mice2005
Author(s)
Nishioka T, Ikawa K, Yu Z, Sugawara Y, Kawagoe J, Takizawa T, Primo V, Nikolic B, Sasano T, Shimauchi H, Takada H, Endo Y, Sugawara S
Organizer
12^<th> International Congress of Mucosal Immunology
Place of Presentation
Boston
Description
「研究成果報告書概要(欧文)」より
Related Report
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