| Project/Area Number |
17591959
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tohoku University |
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Principal Investigator |
SUGAWARA Yumiko Tohoku University, Tohoku University, Hospital, Assistant Professor (30235866)
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| Co-Investigator(Kenkyū-buntansha) |
SHOUJI Noriaki Hospital, 病院, Assistant Professor (70250800)
SUGAWARA Shunji Graduate School of Dentistry, 大学院・歯学研究科, Proffessor (10241639)
SASANO Takashi Graduate School of Dentistry, 大学院・歯学研究科, Proffessor (10125560)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥2,950,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | oral mucosa / The innate immune system / IL-18 / the lesion of the ulcer and erosion / TLRs / NODs / IL-17 / Fas / FasL |
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| Research Abstract |
Oral epithelial cells may form the first barriers of defense against oral bacteria in periodontal tissue. In this study, we thoroughly examined the expression of Toll-like receptor (TLR)2, TLR4, nucleotide-binding oligomerization domain (NOD)l and NOD2, and their possible antibacterial roles in terms of the expression of peptidoglycan recognition proteins (PGRPs) and b-defensin 2 in oral epithelial cells.
1) Oral epithelial cells constitutively express TLR2, TLR4, NOD1 and NOD2 molecules.
2) Oral epithelial cells do not secrete proinflammatory cytokines upon stimulation with synthetic PAMPs.
3) Synthetic PAMPs stimulated human oral epithelial cells to increase the expressions of four lands of PGRPs and b-defensin 2.
4) Up-regulation of PGRPs by Pam3CSSNA, lipid A, iE-DAP, and AMP were performed through TLR2, TLR4, NODI and NOD2, respectively.
5) The up-regulation of PGRPs induced by synthetic PAMPs was significantly inhibited in NF-kB p50- and p65-silenced cells.
6) Inflamed gingival epithelial cells clearly express PGRP proteins.
7) meso-DAP itself activates epithelial cells but not monocytic cells through NOD1.
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