Relationship between the radio-sensitivity and degree on alteration of nuclear proteins in oral cancer apoptotic cell
| Project/Area Number |
17591971
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
MORIMOTO Yasuhiro Kyushu Dental College, Department of Oral Diagnositc Science, Professor (00275447)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | apoptosis / nuclear proteins / AgNORs / nucleolin / protein nhostatase / cleavage / 蛋白質脱リン酸化酵素 / ニュークレオフォスミン |
|---|
| Research Abstract |
Background : To investigate the behavior of nuclear proteins in apoptosis induced by protein phosphatase 1 inhibitors, okadaic acid, anti-cancer drugs, and UV radiation in cultured human oral cancer cells.
Methods : Dynamic alternations of nucleolin and AgNOR proteins in all inducer-induced apoptosis of cultured human oral cancer cells and in a cell-free apoptotic system were examined using Western blot analysis and immunocytochemical method.
Results: The 110-kD_a form of nucleolin and AgNOR protein decreased and the 80-, and 95-kD_a forms appeared during apoptosis in human oral cancer cells and in a cell-free apoptotic system. In addition, the induction of DNA ladder formation coincided with the appearance of alternation of nucleolin and AgNOR proteins in a cell-free apoptosis. Nucleolin diffusely spread out into the nuclear material in the apoptotic body of human oral cancer cells.
Conclusions : The present results indicate that alternations of nucleolin and AgNOR proteins are associated with the induction of DNA fragmentation and the final active phase of apoptosis induced by okadaic acid-, anti-cancer drugs, UV radioation in oral cancer cells.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Functional evaluations of the parotid and submandibular glands using dynamic MR sialography2007
Author(s)
Tanaka T, Ono K, Habu M, Inoue H, Tominaga K, Okabe S, Kito S, Yokota M, Fukuda J, Inenaga K, Morimoto, Y
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Journal Title
Dentomaxillofac Radiol 36
Pages: 218-223
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Clinical significance and variation of the advanced calcified stylohyoid complex detected by panoramic radiographs among 80-year-old subjects2006
Author(s)
Okabe S, Morimoto Y, Ansai T, Yamada K, Tanaka T, Awano S, Kito S, Takata Y, Takehara T, Ohba, T
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Journal Title
Dentomaxillofac Radiol 35
Pages: 191-199
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Can the presence of carotid artery calcification on panoramic radiographs predict the risk of vascular diseases among 80-year-olds2006
Author(s)
Tanaka T, Morimoto Y, Ansai T, Okabe S, Yamada K, Taguchi A, Awano S, Kito S, Takata Y, Takehara T, Ohba, T
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Journal Title
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101
Pages: 777-783
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Dynamic MR sialography as a new diagnostic technique for patients with Sjogren syndrome2006
Author(s)
Morimoto Y, Habu M, Tomoyose T, Ono K, Tanaka T, Yoshioka I, Tominaga K, Yamashita Y, Ansai T, Kito S, Okabe S, Takahashi T, Takehara T, Fukuda J, Inenaga K, Ohba, T
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Journal Title
Oral Dis 12
Pages: 408-414
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Utility of three dimension fast asymmetric spin-echo(3D-FASE)sequences in MR sialographic sequences : model and volunteer studies2005
Author(s)
Morimoto Y, Tanaka T, Kito S, Tominaga K, Yoshioka I, Yamashita Y, Shibuya T, Matsufuji Y, Kodama M, Takahashi T, Fukuda J, Ohba, T
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Description
「研究成果報告書概要(欧文)」より
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