| Project/Area Number |
17591971
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
MORIMOTO Yasuhiro Kyushu Dental College, Department of Oral Diagnositc Science, Professor (00275447)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | apoptosis / nuclear proteins / AgNORs / nucleolin / protein nhostatase / cleavage / 蛋白質脱リン酸化酵素 / ニュークレオフォスミン |
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| Research Abstract |
Background : To investigate the behavior of nuclear proteins in apoptosis induced by protein phosphatase 1 inhibitors, okadaic acid, anti-cancer drugs, and UV radiation in cultured human oral cancer cells.
Methods : Dynamic alternations of nucleolin and AgNOR proteins in all inducer-induced apoptosis of cultured human oral cancer cells and in a cell-free apoptotic system were examined using Western blot analysis and immunocytochemical method.
Results: The 110-kD_a form of nucleolin and AgNOR protein decreased and the 80-, and 95-kD_a forms appeared during apoptosis in human oral cancer cells and in a cell-free apoptotic system. In addition, the induction of DNA ladder formation coincided with the appearance of alternation of nucleolin and AgNOR proteins in a cell-free apoptosis. Nucleolin diffusely spread out into the nuclear material in the apoptotic body of human oral cancer cells.
Conclusions : The present results indicate that alternations of nucleolin and AgNOR proteins are associated with the induction of DNA fragmentation and the final active phase of apoptosis induced by okadaic acid-, anti-cancer drugs, UV radioation in oral cancer cells.
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