| Project/Area Number |
17591993
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OZAKI Kazumi The University of Tokushima, Tokushima University Hospital, Assistant professor, 医学部・歯学部附属病院, 講師 (90214121)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Takashi The University of Tokushima, Institute of Health Biosciences of Tokushima Graduate School, Professor, 大学院ヘルスバイオサイエンス研究部, 教授 (30173800)
HOSOKAWA Yoshitaka The University of Tokushima, Institute of Health Biosciences of Tokushima Graduate School, Instructor, 大学院ヘルスバイオサイエンス研究部, 助手 (90346601)
KAWASAKI Akiko The University of Tokushima, Institute of Health Biosciences of Tokushima Graduate School, Instructor, 大学院ヘルスバイオサイエンス研究部, 助手 (60294708)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Dentinal caries / Streptococcus mutans / Intracellular polysaccharide / Quorum sensing system |
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| Research Abstract |
Streptococcus mutans that is one of the cariogenic bacterium is synthesized the water-soluble glucan (WSG) and the water-insoluble glucan (WIG) from sucrose, adheres on the tooth, and proliferates. In this study, S. mutans wild-type strain UA159 and its knockout mutants, which were defective in comC, comX and scrA mutant, were examined for their ability for initial adhesion, glucan synthesis and invasion into dentinal tubles. The proportions of water-insoluble glucan synthesis in all mutant strains were less than the wild type. The proportions of water-soluble glucan adhering to dentin slices in all mutant strains were also less than in the wild type. The total dose of three kinds of glucan composition decreased in all mutant strains. The expansion rates of dentinal tubules by comC and comX mutant increased more than by the wild type when cultured in brain heart infusion for 24 days. There were no detectable difference in growth between wild-type and scrA-mutant. In the presence of sucrose, the produce of WSG and WIG in scrA-mutant was less than in wild-type. This study demonstrated that the quorum-sensing system and PTS system may play a crucial role in glucan synthesis, biofilm formation, production of intracellular-polysaccharide and invasion to dentinal tubules of S.mutans.
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