| Project/Area Number |
17591997
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
OYAMA Tohru Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor (60233623)
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| Co-Investigator(Kenkyū-buntansha) |
SUWA Motoko Kagoshima university, Medical and Dental Hospital, Assistant Professor (80206599)
TOKUDA Masayuki Kagoshima university, Medical and Dental Hospital, Senior Assistant Professor (20253891)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Apical periodontitis / MMP-1 / Polymorphism / Diaffnosis of risk on apical lesion / Ets-1 / リスク診断 / Ets-1 |
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| Research Abstract |
Transcription factor Ets-1 play critical role in MMP-1 mRNA expression. A single nucleotide polymorphism in the promoter region of -1607 by of 1VIMP-1 have influence on Ets-1 binding activity. To clarify the ability of Ets-1 binding activity, we examined the following experiment.
Methods : Human periodontal ligament cells were plated in cell culture dishes in α-MEM supplemented with 10% FBS. The confluent-stage cells were incubated with 1% FBS containing α-MEM for 24h, followed by treatment with 10 ng/ml TNF-α. After incubation, the culture supernatants and sediments were collected and analyzed by ELISA, Northern blot, and gel shift mobility assay.
Results TNF-α induced MMP-1 mRNA and protein in various HPDL cells, and there were variations slightly in its expression. However, there were no significant differences in analysis of protein production by ELISA. TNF-α-induced ets-1 mRNA and protein also did not differ among HPDL cells, and Ets-lactivation in response to TNF-α showed that there were variations in activations. We are undertaking further investigations in our laboratory to elucidate the precise genetic analysis of polymorphism involved in variations of MMP-1 expression by TNF-α stimulations.
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