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Development of dental biosensor using nano-technology

Research Project

Project/Area Number 17592054
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionOsaka Dental University

Principal Investigator

HASHIMOTO Yoshiya  Osaka Dental University, Dentistry, Instructor, 歯学部, 助手 (20228430)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masaaki  Osaka Dental University, Dentistry, Professor, 歯学部, 教授 (50067055)
KUSUNOKI Masanobu  Kinki university, Biology oriented science and tecnology, Associate professor, 生物理工学部, 助教授 (20282238)
HONTSU Shigeki  Kinki university, Biology oriented science and tecnology, Professor, 生物理工学部, 教授 (40157102)
NISHIKAWA Hiroaki  Kinki university, Biology oriented science and tecnology, lecturer, 生物理工学部, 講師 (50309267)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsquartz crystal microbalance / ArF excimer laser / apatite / biosensor / nano-technology / 水晶振動子マイクロバランス / ハイドロキシパタイト
Research Abstract

This study examined in vitro cytocompatibility and in situ measurements of protein adsorption using the quartz crystal microbalance (QCM) to thin hydroxyl apatite (HA) and natural apatite (NA) films deposited by laser ablation, in order to investigate better surface conformation of titanium dental implants.
Thin HA and NA films on titanium discs and the QCM sensor were made using an ArF excimer laser operating at a repletion rate of 10 Hz and annealed by heat radiation at 360℃ for 1 h. After annealing, the chemical composition of the HA film was characterized by X-ray diffraction (XRD). The surface conformation of the HA and NA films on the titanium disc was observed by energy dispersive analysis (EDX). Subsequently, primary mouse osteoblast (PMO) was cultured on the discs with or without film. Cell proliferation and differentiation were investigated by DNA assay, alkaline phosphatase activity (ALP) assay and osteocalcin production (OCN) assay. The affinity between the films and several proteins in the phosphate buffer was monitored using a 27-Hz QCM.
XRD showed crystalline HA and NA films on the Ti and QCM sensor under the annealing conditions. EDX showed peaks of Na and Mg in addition to Ca and P for the NA film. PMO grew faster and had higher ALP and OCN when grown in NA films than on HA films or Ti. The absorbed amount of several proteins on the QCM sensor with NA film was significantly higher than that of HA film and non-film.
Based on these findings, NA film deposited by laser ablation shows excellent cytocompatibility with cells owing to the superior absorption of protein. The results obtained could lead to the development of more effective dental implants.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (8 results)

All 2006 Other

All Journal Article (8 results)

  • [Journal Article] Improvement of hydroxyapatite deposition on titanium dental implant using ArF laser ablation -It's effect on osteoblast biocompatibility in vitro-2006

    • Author(s)
      HASHIMOTO Y, KUSUNOKI M, HATANAKA R, HAMANO K, NISHIKAWA H, HOSOI Y, HONTSU S
    • Journal Title

      ADVANCES IN SCIENCE AND TECHNOLOGY 49

      Pages: 282-289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 生体アパタイト超薄膜被覆インプラントの生体親和性2006

    • Author(s)
      本津茂樹, 川島将美, 西川博昭, 楠正暢, 速水 尚, 大橋芳夫, 樋口裕一, 橋本典也
    • Journal Title

      バイオマテリアル 24・5

      Pages: 353-360

    • NAID

      10020361645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Biocompatibility of implant with natural apatite ultra-thin film2006

    • Author(s)
      HONTSU S, KAWASHIMA M, NISHIKAWA H, KUSUNOKI M, HAYAMIT, OHASHI Y, HIGUCHI Y, HASHIMOTO Y
    • Journal Title

      Journal of Japanese Society for Biomaterials 24

      Pages: 353-360

    • NAID

      10020361645

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Improvement of hydroxyapatite deposition on titanium dental implant using ArF laser ablation - It's effect on osteoblast biocompatibility in vitro-2006

    • Author(s)
      HASHIMOTO Y, KUSUNOKI M, HATANAKA R, HAMANO K, NISHIKAWA H, HOSOI Y, HONTSU S
    • Journal Title

      ADVANCES IN SCIENCE AND TECHNOLOGY 49

      Pages: 282-289

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 生体アパタイト超薄膜被覆インプラントの生体親和性2006

    • Author(s)
      本津茂樹, 川島将美, 西川博昭, 楠 正暢, 速水 尚, 大橋芳夫, 樋口裕一, 橋本典也
    • Journal Title

      バイオマテリアル 24・5

      Pages: 353-360

    • NAID

      10020361645

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser

    • Author(s)
      Y.HASHIMOTO, M.KAWASHIMA, R.HATANAKA, M.KUSUNOKI, H.NISHIKAWA, S.HONTSU, M.NAKAMURA
    • Journal Title

      J Mater Sic Mater in Med (impress)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser

    • Author(s)
      Y.HASHIMOTO, M.KAWASHIMA, R.HATANAKA, M.KUSUNOKI, H.NISHIKAWA, S.HONTSU, M.NAKAMUR
    • Journal Title

      J Mater Sic Mater in Med (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser

    • Author(s)
      Y.HASHIMOTO, M.KAWASHIMA, R.HATANAKA, M.KUSUNOKI, H.NISHIKAWA, S.HONTSU, M.NAKAMURA
    • Journal Title

      J Mater Sic Mater in Med (impress)

    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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