• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation of pre-operative Chemoradiotherapy in Oral Squamous Cell Carcinoma. -Integration of molecular biology and clinical state-

Research Project

Project/Area Number 17592062
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHokkaido University

Principal Investigator

OHIRO Yoichi  Hokkaido University, Graduate School of Dental Medicine, Instructor, 大学院歯学研究科, 助手 (40301915)

Co-Investigator(Kenkyū-buntansha) SHINDOH Masanobu  Hokkaido University, Graduate School of Dental Medicine, Professor, 大学院歯学研究科, 教授 (20162802)
KOBAYASHI Masanobu  Hokkaido University, Institute for Genetic Medicine, Associate Professor, 遺伝子病制御研究所, 助教授 (80241321)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsOral Cancer / Radiation therapy / Chemotherapy / p53 / Genetic alteration / Hypoxia / 治療効果予測因子
Research Abstract

The predictive value of tumor suppressor protein of p53 and related genes for the effect of chemoradiation therapy on oral squamous cell carcinoma is still controversial. Recently, it is clear that tumor tissues are exposed to low-oxygen environmental (hypoxic state) so tumor cells alter their gene expression profile to survive in a severe condition. We estimate the proteins expression for p53 and p53R2 (p53-dependent kinase gene) in biopsy specimen to examine these proteins are valuable predictors for concurrent chemoradiotherapy. We also examined hypoxia-inducible factor (HIF) responsible proteins, vascular endothelial growth factor (VEGF) and Angiopoietin-like4 (ANGPTL4) in vitro to understand their relation under hypoxic state.
Thirty five cases were enrolled in this study. Fifteen patients were received radiation therapy only and 20 patients were given concurrent chemoradiotherapy before the operation. Biopsy specimens were examined by immunohistochemistry. The positive rate of p53 was 80% and p53R was 73.3 in all patients. Statistical anaylysis revealed that the combination with p53 positive/negative and p53R positive/negative couldn't predict the sensitivity to preoperative concurrent chemoradiotherapy.
In vitro study, VEGF and hypoxic condition stimulate the expression for ANGPTL4. Under hypoxic condition, over expression of ANGPTL4 down-regulates the expression of VEGF. These results suggest that under hypoxic condition, VEGF expression is negatively regulated by ANGPLT4.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (2 results)

All 2006

All Journal Article (2 results)

  • [Journal Article] 低酸素下におけるVEGFとAngiopoietin-like4の相互制御機構2006

    • Author(s)
      鎌田 卓
    • Journal Title

      北海道歯学雑誌 Vol.27

      Pages: 96-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cross- regulation of VEGF and Angiopoietin-like4 under hypoxia.2006

    • Author(s)
      Kamata S, Matsuizaka M, Nakagawa K, Ohiro Y, Shindoh M, Higashino F, Koide F, Kobayashi M, Totsuka Y
    • Journal Title

      Hokkaido Journal of Dental Science 27

      Pages: 96-103

    • NAID

      10018675950

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi