| Co-Investigator(Kenkyū-buntansha) |
SATOH Hisashi Hirosaki University, University Hospital, Instructor, 医学部附属病院, 助手 (90311539)
SASAKI Hirotaka Hirosaki University, University Hospital, Instructor, 医学部附属病院, 助手 (90374850)
KUSUMI Tomomi Hirosaki University, School of Medicine, Lecturer, 医学部, 講師 (90322932)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
1.Effects of bone-related factors on human osteoblasts with the application of cyclic tensile strain (CTS)
It was suggested that the synthesis of OPG and RANKL from human osteoblasts might regulate via p38 MAPK because of results of analyses of p38MAPK inhibitor, SB250380,and real-time RT-PCR and ELISA. The application of CTS enhanced synthesis of OPN, the expression of Cox-2 mRNA, and NO production.
2.Analyses of signal transduction in human osteoblasts with the application of CTS
We used with a DNA array method, Sentrix^<TM> Human-6 Expression BeadChip. The application of CTS enhanced the mRNA expressions of Ras families, TNF receptor families, tubulin families, and so on, not enhancing the mRNA expressions of NF-κB、Runx2/Cbfal、Osterix、Msx、Dlx5、Smad families, and so on.
3.Analyses on mouse osteosarcoma, NHOS, with application of CTS
We could examine the analyses on mouse osteosarcoma, NHOS, with application of CTS because of the breakout of harmful insects in the animal experimental facul
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ty in Hirosaki University School of Medicine.
4.The relation between osteoblasts with the application of CTS and association with ras gene
Synthesis of OPG from osteoblasts enhanced as maintaining culture passages without the application of CTS. Though enhancing synthesis of OPG from osteoblasts at the 1^<st> and 2^<nd> passages, the application of CTS inhibited that at the 3rd passages. Though the levels of sRANKL release from osteoblasts were not different between the 1^<st> passage and 3^<rd> passage, the application of CTS inhibited the expression of RANKL in osteoblasts at the 1^<st> passage and enhanced in osteoblasts at the 3^<rd> passage. The synthesis of OPN, the expression of Cox-2, and NO production enhanced from osteoblasts at the 1^<st> and 3^<rd> passages. Activation of p38 MAPK and inactivation of ERK1/2 in osteoblasts at the 1^<st> passage induced 15 minutes after the beginning of the application of CTS, and inactivation of p38 MAPK and activation of ERK1/2 in osteoblasts at the 3^<rd> passage induced 15 minutes after the beginning of the application of CTS.
5.Conclusion
These results will be suggested to find new evidences in future. Less
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