| Project/Area Number |
17592069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
TAKATSUKA Shigeyuki Kanazawa University, Universty Hospital, Assistant Professor (00251926)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Kiyomasa Kanazawa University, Graduate School of Medical Science, Associate Professor (30155676)
YAMAMOTO Etsuhide Kanazawa University, Graduate School of Medical Science, Professor (00092445)
HUKUDA Ryuji Kanazawa University, Graduate School of Medical Science, Associate Researcher (60027331)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,590,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥90,000)
Fiscal Year 2007: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | temporomandibular disorder / articular disc / synovial fluid / aggrecanase(ADAMTS) / matrix metalloproteinase(MMP) / fibrous adhesion |
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| Research Abstract |
1. Experimental study of temporomandibular joint arthritis in the rabbits
Proteolytic activities of the temporomandibular arthritis have been investigated using a rabbit model of temporomandibular disorders. Histopathologic examination has been achieved on the arthritis caused by an artificial dislocation of the articular disc. Throughout the examination period, histoimmunochemical staining showed expression of proteolytic enzymes such as matrix metalloproteinases (MMPs) and aggrecanase(ADAMTS). Especially in the critical destructive period, specimens showed higher incidence of enzymatic expression. Expression of type I and H collagen stain were also noticed in the cartilage layer of the mandibular condyle. Distribution of these components differed according to the destruction periods.
2. Analyses of the clinically harvested materials
Histopatholigic and biochemistric examination have been carried out using human materials of synovial fluid and biopsy tissue from the upper joint compartment of the temporomandibular joints, under informed consent. In patients with prominent arthritic changes on Milk showed higher incidence of MMP5 and ADAMTS. During cartilage matrix destruction, ADAMTS first acts on proteoglycan breakdown, then MMPs act on proteoglycan and collagen breakdown. These enzymatic expressions have been noticed in RA patients in the field of Orthopedic Surgery. Conversely in the present study, high incidence of both enzymatic activities has been noticed in the temporomandibular joints. From the results, arthritis may be mild in the temporomandibular joints. Harvested adhesion tissue via arthroscopic surgery showed a number of fibro-blastic cells. Specimens from advanced stage of arthritis showed ossification or cartilaginous degeneration. These lesions are frequently showed in preoperative MRI images.
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