Molecular analysis of tooth regeneration

• Project/Area Number: 17592072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyoto University
• Principal Investigator: TAKAHASHI Kastuji Kyoto University, Graduate School of Medicine, Lecturer (90314202)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Akira Kyoto University, Graduate School of Medicine, Professor (00162694) ; SUGAI Manabu Kyoto University, Graduate School of Medicine, lecturer (90303891)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: tooth regeneration / USAG-1 / diphyodont / polymorphism / 器官培養 / 組み換えウイルス

Research Abstract

Uterine sensitization associated gene-1 (USAG-1) is a BMP antagonist that functions in the local regulation of BMP mediated signal transduction by binding and neutralizing BMP activities, and also modulates Wnt signaling. We previously reported that USAG-1 deficient mice have supernumerary teeth. The supernumerary maxillary incisor appears to form as a result of the successive development of the rudimentary upper incisor. USAG-1 abrogation rescued apoptotic elimination of odontogenic mesenchymal cells. We confirmed that BMPs were expressed in both the epithelium and mesenchyme of the rudimentary incisor at E14 and E15. BMP signaling in the rudimentary maxillary incisor, assessed by expressions of Msx1 and Dlx2 and the phosphorylation of Smad protein, was significantly enhanced. Inhibition of BMP signaling rescues supernumerary tooth formation in E15 incisor explant culture. Based upon these results, we conclude that enhanced BMP signaling results in supernumerary teeth in the USAG-1 deficient mouse model.

Research Products

• [Journal Article] Enhanced BMP signaling results in supernumerary tooth formation in USAG-1 deficient mouse (2008)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomoko Sakata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C.Slavkin and kazuhisa Bessho
  • Journal Title: Biochem Biophys Res Commun (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhanced BMP signaling results in supernumerary tooth formation in USAG-1 deficient mouse. (2008)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomoko Sakata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C. Slavkin, Kazuhisa Bessho.
  • Journal Title: Biochem Biophys Res Commun (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rudiment incisors survive and erupt as supernumerary teeth as a result of USAG-1 abrogation (2007)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomohiro Kawabata, Tomoko Skata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Skurai, Harold C.Slavkin and Kazuhisa Bessho
  • Journal Title: Biochem Biophys Res Commun 359 Pages: 549-555
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rudiment incisors survive and erupt as supernumerary teeth as a result of USAG-1 abrogation. (2007)
  • Author(s): Akiko Murashima-Suginami, Katsu Takahashi, Tomohiro Kawabata, Tomoko Sakata, Hiroko Tsukamoto, Manabu Sugai, Motoko Yanagita, Akira Shimizu, Takeshi Sakurai, Harold C. Slavkin, Kazuhisa Bessho.
  • Journal Title: Biochem Biophys Res Commun 359 Pages: 549-555
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Uterine sensitization-associated gene-1 (USAG-1),a novel BMP antagonist experssed in the kidney,accelerates tubular injury (2006)
  • Author(s): Yanagita M., Okuda, T., Endo, S., Tanaka, M., Takahashi, K., Sugiyama, F., Kunita, S., Takahashi, S., Fukatsu, A., Yanagisawa M., Kita, T.and Sakurai T.
  • Journal Title: J Clin Invset 116 Pages: 70-79
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Uterine sensitization-associated gene-1(USAG-1), a novel BMP antagonist expressed in the kidney, accelerates tubular injury. (2006)
  • Author(s): Yanagita M., Okuda, T., Endo, S., Tanaka, M., Takahashi, K., Sugiyama, F., Kunita, S., Takahashi, S., Fukatsu, A., Yanagisawa M., Kita, T., Sakurai T.
  • Journal Title: J Clin Invset. 116 Pages: 70-79
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Uterine sensitization-associated gene-1 (USAG-1), a novel BMP antagonist expressed in the kidney, accelerates tubular injury (2006)
  • Author(s): Yanagita M., Okuda, T., Endo, S., Tanaka, M., Takahashi, K., Sugiyama, F., Kunita, S., Takahashi, S., Fukatsu, A., Yanagisawa M., Kita, T., Sakurai T
  • Journal Title: J Clin Invset. 116 Pages: 70-79
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cellcarcinoma syndrome (2005)
  • Author(s): Tanioka, M., Takahashi, K., Kosugi, kawabata, T., Nishigori, C., Miyachi, Y., and Iizuka, T.
  • Journal Title: Arch.Dermatol.Res 296 Pages: 303-308
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblatstoma (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami, A., Ando, S., Shumizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K.and Iizuka, T.
  • Journal Title: J.Dent.Res 84 Pages: 812-816
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblastoma. (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami, A., Ando, S., Shumizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K., lizuka, T.
  • Journal Title: J. Dent. Res. 84 Pages: 812-816
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome (2005)
  • Author(s): Tanioka, M., Takahashi, K., Kosugi, S., Kawabata, T., Nishigori C., Miyachi, Y., Iizuka, T.
  • Journal Title: Arch.Dermatol.Res 296 Pages: 303-308
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblastoma. (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami A., Ando, S., Shumizu A., Kosugi, S., Sato, T., Nishida M., Murakami, K., Iizuka, T.
  • Journal Title: J.Dent.Res 84 Pages: 812-816
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome
  • Author(s): Tanioka, M., Takahashi, Kosugi, S., Kawabata, T., Nishigori C., Miyachi, Y., Iizuka, T.
  • Journal Title: Arch. Dermatol. Res. 296 Pages: 303-308
  • Description: 「研究成果報告書概要(欧文)」より