| Project/Area Number |
17592082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
FUKUI Yasuto Hiroshima University, Hospital, Assistant, 病院, 医員 (90363085)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Tetsuji Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (00169153)
YAN Zhang Hiroshima University, Graduate School of Biomedical Sciences, Assistant, 大学院医歯薬学総合研究科, 助手 (50332797)
KOBAYASHI Masashi Hiroshima University, Graduate School of Biomedical Sciences, Assistant, 大学院医歯薬学総合研究科, 助手 (30346506)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | animal cap / tooth induction / jaw |
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| Research Abstract |
In this study, we had the following knowledge.
1) We have achieved long-term animal cap culture in a nutrient-supplemented culture medium designated RDX. The explants cultured in RDX grew more vigorously compared with those in Steinberg's solution, and also increased the frequency of cartilage induction. This long-term culture method using RDX medium is useful for studying the differentiation of organs as cartilage in vitro.
2) To induce jaw cartilage from undifferentiated cells effectively, we developed a culture method to manipulate body patterning in vitro, using activin A and dissociated animal cap cells. An aggregate consisting only activin A-treated dissociated cells developed into endodermal tissues. However, when activin A-treated cells mixed with untreated cells at a ratio of 1:5, the aggregate develop cartilage. The frequency of the aggregate that formed cartilage tissue was 100%. This cartilage was positive in Whole mount Alcian blue staining, and we could extract cartilage tissue from explants.
3) To confirm whether the maxillofacial region was induced in the 1:5 aggregates, we further examined the gene expression of goosecoid (gsc), Xenopus distal-less 4 (X-dll 4), Sox 9, and Collagen type 2 (Co12). Analysis using RT-PCR showed that all these gene expression were detected in the 1:5 aggregates. These results indicated that the 1:5 aggregates gave rise to maxillofacial derivatives.
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