• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Association of aberrant receptor tyrosine kinase signaling pathways in malignant transformation of endothelial cells

Research Project

Project/Area Number 17592086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

WANG Hua  HIROSHIMA UNIVERSITY, Graduate school of Biomedical Science, Assistant, 大学院医歯薬学総合研究科, 助手 (50363081)

Co-Investigator(Kenkyū-buntansha) ZHANG Yan  HIROSHIMA UNIVERSITY, Graduate School of Biomedical Science, Assistant, 大学院医歯薬学総合研究科, 助手 (50332797)
YOSHIKO Yuji  HIROSHIMA UNIVERSITY, Graduate School of Biomedical Science, Associate Professor, 大学院医歯薬学総合研究科, 助教授 (20263709)
MAEDA Norihiko  HIROSHIMA UNIVERSITY, Graduate School of Biomedical Science, Professor, 大学院医歯薬学総合研究科, 教授 (60049418)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsTie2 / Angiopoietin / Haemangioma / Angiosarcoma / Fibrous growth factor 23 / Hemangioma / Angiosarcoma
Research Abstract

Tie2, an endothelium-specific receptor tyrosine kinase, plays a central role in controlling vascular homeostasis. We previous findings that a mutation G833D in ATP-binding domain of Tie2 (Tie2_<G833D>) from human benign haemangiomas has mitogenic and transforming potentialities are extended by the current study. Indeed, murine vascular endothelial cell lines overexpressing Tie2_<G833D> (MSS31G833D) displayed the typical features of neoplastic transformation: the cell lost cell-cell contact inhibition, increased the expressions of PCNA (proliferating cell nuclear antigen), and angiosarcoma developed in nude mice. Notably, We found that Ang2 could effectively block Ang1 activation of wild-type Tie2 and Q837H Tie2, but failed to inhibit Ang1-induced Tie2G833D autophosphorylation. Osteomalacia often occurs in vascular tumors, including haemangioma and angiosarcoma. It has been reported that fibroblast growth factor 23 (FGF23), a secreted peptide hormone, overproduced in patients with tumor … More induced osteomalacia. Thus, we examined expression of FGF23 in these transfectants. Real-time PCR showed that MSS31G833D cells expressed FGF23 mRNA more than does control cells. To invest role of FGF23 in osteomalacia, we constructed recombinant adenovirus-human FGF23 (AV-FGF23) by using the Adeno-X^<TM> expression system and assessed the effects of FGF23 overexpression during osteoblast development and matrix mineralization in the RC model. Infection of proliferating cells with AV-FGF23 did not significantly affect cell growth as compared to cells infected with control adenovirus. However, we found that cells overexpressing FGF23 made fewer and smaller nodules than control cultures with a parallel decrease in levels of osteoblast differentiation marker mRNAs. Furthermore, FGF23 overexpression inhibited osteoid nodule mineralization in the presence of β-glycerophosphate. Thus, we conclude that G833D mutant may escape the negative regulation of Ang2 on the receptor and result in elevation of FGF23 expression. FGF23 may act as a local negative regulator of osteoblast development and matrix mineralization. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (17 results)

All 2007 2006 2005

All Journal Article (16 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Mineralized tissue cells are a principal source of FGF23.2007

    • Author(s)
      Yoshiko Y., Wang H., et al.
    • Journal Title

      Bone (Epub ahead of print)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mineralized tissue cells are a principal source of FGF23.2007

    • Author(s)
      Yoshiko Y., Wang H., Minamizaki T., Ijuin C., Yamamoto R, Suemune S., Kozai K., Tanne K., Aunin J.E., Maeda N.
    • Journal Title

      Bone (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mineralized tissue cells are a principal source of FGF23.2007

    • Author(s)
      Yoshiko Y., Wang H., et al.
    • Journal Title

      Bone (掲載決定)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Development Signaling Disorders in Craniofacial Anomalies and Cancers.2006

    • Author(s)
      Zhang Y., Wang H., et al.
    • Journal Title

      Oral Science International 3(2)

      Pages: 53-63

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FGF23 acts as an autocrine/paracrine negative regulator for osteoblast development and matrix mineralization in fatal rat calvaria cell cultures.2006

    • Author(s)
      Wang H., Yamamoto R et al.
    • Journal Title

      J. Bone Miner. Res. 21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Developmental Signaling Disorders in Craniofacial Anomalies and cancers.2006

    • Author(s)
      Zhang Y., Wang H., Kamegai A., Hata T., Kitamura N., Hosoda M., Tani R., Hayashido Y, Toratani S., Okamoto T.
    • Journal Title

      Oral Science International 3(2)

      Pages: 56-63

    • NAID

      10020357220

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FGF23 acts as an autocrine/paracrine negative regulator for osteoblast development and matrix mineralization in fetal rat calvaria cell cultures.2006

    • Author(s)
      Wang H., Yamamoto R., Minamizaki T., Suemune S., Kozai K., Tanne K., Aunin J.E., Maeda N., Yoshiko Y.
    • Journal Title

      J Bone Miner.Res. 21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Remedies for mineralization regulation.2006

    • Author(s)
      Yoshiko Y., Wang H., Maeda N., Yamamoto R, Minamizaki T., Yoshida B.
    • Journal Title

      Patent : Japan 2006

      Pages: 194078-194078

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development Signaling disorders in Craniofacial Anomalies and Cancers.2006

    • Author(s)
      Zhang Y., Wang H., et al.
    • Journal Title

      Oral Science International 3(2)

      Pages: 53-63

    • Related Report
      2006 Annual Research Report
  • [Journal Article] FGF23 acts as an autocrine/paracrine negative regulator for osteoblast development and matrix mineralization in fetal rat calvaria cell cultures.2006

    • Author(s)
      Wang, H., Yamamoto R et al.
    • Journal Title

      J. Bone Miner. Res. 21

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Constitutive activating mutation of the FGFR3b in oral squamous cell carcinomas2005

    • Author(s)
      Zhang Y., Wang H., et al.
    • Journal Title

      International Journal Cancer 117

      Pages: 166-168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adenovirus-mediated overexpression of FGF23 suppresses osteoblast development and matrix mineralization in fetal rat calvaria cell.2005

    • Author(s)
      Wang H., Yamamoto R et al.
    • Journal Title

      J. Bone Miner. Res. 20

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Constitutive activating mutation of the FGFR3b in oral squamous cell carcinomas.2005

    • Author(s)
      Zhang Y., Hiraishi Y, Wang H.Sato J.D., Okamoto T.
    • Journal Title

      Int.J Cancer 117

      Pages: 166-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adenovirus-mediated overexpression, of FGF23 suppresses osteoblast development and matrix mineralization in fetal rat calvaria cell.2005

    • Author(s)
      Wang H., Yamamoto R., Minamizaki T., Suemune S., Kozai K., Tanne K., Aunin J.E., Maeda N., Yoshiko Y.
    • Journal Title

      J.Bone Miner.Res. 20

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Constitutive activating mutation of the FGFR3b in oral squamous cell carcinomas2005

    • Author(s)
      Yang Zhang
    • Journal Title

      International Journal Cancer 117

      Pages: 166-168

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Adenovirus-mediated overexpression of FGF23 suppresses osteoblast development and matrix mineralization in fetal rat calvaria cell.2005

    • Author(s)
      Hua Wang
    • Journal Title

      Journal of Bone and Mineral Research 20 Suppl

    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 石灰化調節剤およびそのスクリーニング法2006

    • Inventor(s)
      吉子裕二, 汪 華, 前田憲彦
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Number
      2006-194078
    • Filing Date
      2006-07-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi